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AlphaLISA Human GAD67 Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for human GAD67 enables the quantitative determination of human glutamate decarboxylase 67 (hGAD67) in serum and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows less than 5% cross reactivity with mouse GAD67. Cross-reactivity with other species has not tested.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL397HV
Unit Size: 100 Assay Points
Part Number: AL397C
Unit Size: 500 assay points
Part Number: AL397F
Unit Size: 5,000 Assay Points
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    Glutamic acid decarboxylase 67 (GAD67) is an enzyme that catalyzes more than 90% of γ-amino butyric acid by glutamate decarboxylation. GAD67 is expressed in the brain and must be active at all times for normal cellular functioning. Dysregulation of GAD67 in the hippocampal stratum oriens layer correlates to the pathophysiology of schizophrenia, bipolar disorders, and Parkinson’s. This enzyme, as well as GAD65, is a target of autoantibodies in humans who develop type 1 diabetes later in their life. This AlphaLISA detection kit allows for the detection of GAD67 levels in serum, plasma, and cell culture supernatants.

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Analysis & Detection
    Assay Points
    500
    Assay Target Class
    Protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Quantity
    1
    Shipping Conditions
    Shipped in Blue Ice
    Therapeutic Area
    Central Nervous System
    Unit Size
    500 assay points