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AlphaLISA Human BACE-1 Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for human BACE-1 enables the quantitative determination of human BACE-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3033F
Unit Size: 5,000 Assay Points
Part Number: AL3033HV
Unit Size: 100 Assay Points
Part Number: AL3033C
Unit Size: 500 Assay Points
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

    Beta-secretase 1 (BACE-1) is an aspartic protease encoded by the BACE-1 gene that is expressed ubiquitously throughout the body. BACE-1 has been identified as a necessary enzyme that functions with gamma-secretase to process of the amyloid precursor protein into the amyloid beta peptide, where the BACE-1 enzyme is responsible for the generation of the variable sizes of the peptide, particularly amyloid beta 1-40 and 1-42. As such, BACE1 is thought to be a valuable biomarker for Alzheimer’s disease, especially in the CSF. This AlphaLISA kit allows for the detection and quantification of BACE-1 in human serum, CSF, cell culture media and cell lysates.

  • Application
    Protein Analysis & Detection
    Assay Points
    5000
    Assay Target
    BACE-1
    Assay Target Class
    Biomarker
    Protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Therapeutic Area
    Oncology
    Unit Size
    5,000 Assay Points