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PhenoVue DAPI nuclear stain

Name: PhenoVue DAPI nuclear stain
Price: 310.99 USD
Availability: InStock

HeLa cells stained with PhenoVue DAPI nuclear stain

PhenoVue™ DAPI nuclear stain is a cell-impermeable organic molecule that binds to DNA and is commonly used to stain cell' nucleus.

Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue DAPI nuclear stain has a maximum excitation wavelength of 348nm and a maximum emission wavelength of 457nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information

Feature	Specification
Color	Blue
Filter	DAPI
Organelle and Cell Compartment	Nucleus

Request more information

PhenoVue™ DAPI nuclear stain is a cell-impermeable organic molecule that binds to DNA and is commonly used to stain cell' nucleus.

Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue DAPI nuclear stain has a maximum excitation wavelength of 348nm and a maximum emission wavelength of 457nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information

Product variant

Quantity: 1 x 1 mg

Part #:

CP81

List price

USD 310.99

Your online price:

For research use only. Not for use in diagnostic procedures.

Product information

Overview

PhenoVue DAPI nuclear stain is a cell-impermeable organic molecule that binds preferentially to adenine-thymine (A-T) regions of minor groove of DNA. When excited by ultraviolet light, DAPI exhibits strong fluorescence at 455 nm. It is commonly used to stain cells’ nuclear compartment.

It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

Typical working concentration: 1 µg/mL (10 nM)

Equivalent number of microplates:

350 - 1040 x 96-well microplates
290 - 1040 x 384-well microplates
540 - 1630 x 1536-well microplates

Specifications

Physical specifications

Color	Blue
Concentration	1 mg/mL (2.85 mM)
Form	Solution in dd H2O
Maximum Emission Wavelength (Em_max)	457 nm
Maximum Excitation Wavelength (Ex_max)	348 nm

Other specifications

Application	High Content Imaging Microscopy
Brand	PhenoVue™
Detection Modality	Fluorescence
Filter	DAPI
Organelle and Cell Compartment	Nucleus
Quantity	1 x 1 mg
Sample Type	Fixed samples only
Shipping Conditions	Shipped Ambient
Storage Conditions	2-8 °C for short term (6 months) or -16 °C or below for long term (> 6 months), protected from light
Type	Individual reagent