PhenoVue™ DAPI nuclear stain is a cell-impermeable organic molecule that binds to DNA and is commonly used to stain cell' nucleus.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue DAPI nuclear stain has a maximum excitation wavelength of 348nm and a maximum emission wavelength of 457nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
For research use only, not for use in diagnostic procedures.
PhenoVue DAPI nuclear stain is a cell-impermeable organic molecule that binds preferentially to adenine-thymine (A-T) regions of minor groove of DNA. When excited by ultraviolet light, DAPI exhibits strong fluorescence at 455 nm. It is commonly used to stain cells’ nuclear compartment.
It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Numbers of vials per unit | 1 |
---|---|
Quantity or volume per unit | 1 mg (1mg/mL - 2.85mM) |
Form | Liquid |
Storage | -20°C |
Recommended working concentration | 1µg/mL (10nM) |
Maximum excitation wavelength | 358 nm |
Maximum emission wavelength | 457 nm |
Common filter set | DAPI |
Live cell staining | usually not, except at high concentrations |
Fixed cell staining | Yes |
Equivalent number of microplates | 350-1040 x 96-well microplates 290-1040 x 384-well microplates 540-1630 x 1536-well microplates |
Color |
Blue
|
---|
Brand |
PhenoVue™
|
---|---|
Detection Method |
Fluorescence
|
Filter |
DAPI
|
Organelle and Cell Compartment |
Nucleus
|
Product Compatibility |
Fixed samples only
|
Shipping Conditions |
Shipped Ambient
|
Type |
Individual Reagent
|
Unit Size |
1 vial
|
This flyer describes Revvity's PhenoVue cellular imaging reagents.
This is the product information sheet for PhenoVue DAPI Nuclear Stain.
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