| Feature | Specification |
|---|---|
| Color | Blue |
| Filter | DAPI |
| Organelle and Cell Compartment | Nucleus |
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PhenoVue DAPI nuclear stain is a cell-impermeable organic molecule that binds preferentially to adenine-thymine (A-T) regions of minor groove of DNA. When excited by ultraviolet light, DAPI exhibits strong fluorescence at 455 nm. It is commonly used to stain cells’ nuclear compartment.
It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Typical working concentration: 1 µg/mL (10 nM)
Equivalent number of microplates:
| Color |
Blue
|
|---|---|
| Concentration |
1 mg/mL (2.85 mM)
|
| Form |
Solution in dd H2O
|
| Maximum Emission Wavelength (Emmax) |
457 nm
|
| Maximum Excitation Wavelength (Exmax) |
348 nm
|
| Application |
High Content Imaging
Microscopy
|
|---|---|
| Brand |
PhenoVue™
|
| Detection Modality |
Fluorescence
|
| Filter |
DAPI
|
| Organelle and Cell Compartment |
Nucleus
|
| Quantity |
1 x 1 mg
|
| Sample Type |
Fixed samples only
|
| Shipping Conditions |
Shipped Ambient
|
| Storage Conditions |
2-8 °C for short term (6 months) or -16 °C or below for long term (> 6 months), protected from light
|
| Type |
Individual reagent
|
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This flyer describes Revvity's PhenoVue cellular imaging reagents.
This is the product information sheet for PhenoVue DAPI Nuclear Stain.
PhenoVue reagents & imaging microplates product list
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