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HTRF Anti-Phospho-PKA substrate pAb Eu-Conjugate, 500 Assay Points
HTRF Anti-Phospho-PKA substrate pAb Eu-Conjugate, 500 Assay Points
Generic HTRF PAb EuK image
| Feature | Specification |
|---|---|
| Application | Biochemical Enzymatic Assay |
Product variants
Unit Size: 500 assay points
Part #:
61P01KAE
List price
USD 923.39
Your online price:
Unit Size: 10,000 assay points
Part #:
61P01KAZ
List price
USD 3,259.70
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Anti-Phospho-PKA substrate pAb Eu-Conjugate, 500 Assay Points
Generic HTRF PAb EuK image
HTRF Anti-Phospho-PKA substrate pAb Eu-Conjugate, 500 Assay Points
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Product information
Overview
The Anti-phospho PKA-Eu cryptate is a labeled polyclonal antibody enabling the detection of peptides and proteins containing a phosphorylated Ser/Thr residue with Arginine in the 3 position. It does not detect the non-phosphorylated PKA. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrates can be used, from small synthetic peptides, to natural substrates for a high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.
How it works
Assay principle
HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.
Assay protocol
HTRF kinase assay have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and PAb Anti-phospho PKA substrate-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.
Specifications
| Application |
Biochemical Enzymatic Assay
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Fluorescent Reagent
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Kinase
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Inflammation
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Anti-Phospho-PKA substrate pAb Eu-Conjugate, 500 Assay Points
HTRF Anti-Phospho-PKA substrate pAb Eu-Conjugate, 500 Assay Points
Resources
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Brochure
HTRF assays and reagents product list
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
Guide
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