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HTRF Anti-Phospho-PKA substrate pAb Eu-Conjugate, 500 Assay Points

PAb Anti-phospho PKA substrate-Eu cryptate enables the detection of phosphorylation of peptides or proteins on Ser/Thr in a biochemical kinase assay format.

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Feature	Specification
Application	Biochemical Enzymatic Assay

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PAb Anti-phospho PKA substrate-Eu cryptate enables the detection of phosphorylation of peptides or proteins on Ser/Thr in a biochemical kinase assay format.

View product information

Product Variants

Part #:

61P01KAE

List Price

USD 923.39

Your online price:

Part #:

61P01KAZ

List Price

USD 3,259.70

Your online price:

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

Product information

Overview

The Anti-phospho PKA-Eu cryptate is a labeled polyclonal antibody enabling the detection of peptides and proteins containing a phosphorylated Ser/Thr residue with Arginine in the 3 position. It does not detect the non-phosphorylated PKA. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrates can be used, from small synthetic peptides, to natural substrates for a high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.

Specifications

Other Specifications

Application	Biochemical Enzymatic Assay
Brand	HTRF
Detection Modality	HTRF
Molecular Modification	Phosphorylation
Product Group	Fluorescent Reagent
Shipping Conditions	Shipped in Dry Ice
Target Class	Kinase
Technology	TR-FRET
Therapeutic Area	Cardiovascular Infectious Diseases Inflammation Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size	500 assay points

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How it works

Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

HTRF PAb Anti-phospho PKA substrate-Eu cryptate 1.svg

Assay protocol

HTRF kinase assay have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and PAb Anti-phospho PKA substrate-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.