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PhenoVue Hoechst 33342 Nuclear Stain 10mg

Name: PhenoVue Hoechst 33342 Nuclear Stain 10mg
Price: 61 USD
Availability: InStock

HeLa cells stained with PhenoVue Hoechst 33342 Nuclear Stain

PhenoVue Hoechst 33342 nuclear stain is a cell-permeable organic molecule that binds to DNA.

PhenoVue Hoechst 33342 nuclear stain exhibits bright blue fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, the PhenoVue Hoechst 33342 nuclear stain has a maximum excitation wavelength of 357 nm and a maximum emission wavelength of 455 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information

Feature	Specification
Color	Blue
Filter	DAPI
Organelle and Cell Compartment	Nucleus

Request more information

PhenoVue Hoechst 33342 nuclear stain is a cell-permeable organic molecule that binds to DNA.

PhenoVue Hoechst 33342 nuclear stain exhibits bright blue fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, the PhenoVue Hoechst 33342 nuclear stain has a maximum excitation wavelength of 357 nm and a maximum emission wavelength of 455 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information

Product variants

Quantity: 1 x 10 mg

Part #:

CP71

List price

USD 61.00

Your online price:

Quantity: 1 x 100 mg

Part #:

CP72

List price

USD 118.00

Your online price:

For research use only. Not for use in diagnostic procedures.

Product information

Overview

PhenoVue Hoechst 33342 Nuclear stain (trihydrochloride form) is a cell-permeable organic molecule that binds preferentially to adenine-thymine (A-T) regions of the minor groove of DNA. When excited by ultraviolet light, Hoechst 33342 nuclear stain exhibits a strong fluorescence at 455 nm. It is commonly used to stain cells' nuclear compartment.

PhenoVue Hoechst 33342 Nuclear stain can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

Typical working concentration: 2 µg/mL (3.24 µM)

Equivalent number of microplates:

170 - 520 x 96-well microplates
140 - 520 x 384-well microplates
270 - 800 x 1536-well microplates

See Product Information sheet for more information on fixed cell staining.

Specifications

Physical specifications

Color	Blue
Concentration	10 mg/mL (16.23 mM)
Form	Solution in dd H2O
Maximum Emission Wavelength (Em_max)	455 nm
Maximum Excitation Wavelength (Ex_max)	357 nm

Other specifications

Application	High Content Imaging Microscopy
Brand	PhenoVue™
Detection Modality	Fluorescence
Filter	DAPI
Organelle and Cell Compartment	Nucleus
Quantity	1 x 10 mg
Sample Type	Live and fixed samples
Shipping Conditions	Shipped Ambient
Storage Conditions	2-8 °C for short term (6 months) or -16 °C or below for long term (> 6 months), protected from light
Type	Individual reagent