PhenoVue Cell Painting Target Add-on Kit (anti-mouse 400LS), 1 x 384

Cell painting is a phenotypic high-content imaging approach that combines multiplexed fluorescent staining, automated microscopy, and image analysis to characterize cellular responses to perturbagens such as compounds, drugs, or genes.

The PhenoVue Cell Painting Target Add-on Kit (anti-mouse 400 LS) extends cell painting workflows by enabling integration of a user-selected mouse primary antibody into a multiparametric phenotypic profiling assay. The selected primary antibody is detected using the PhenoVue Fluor 400LS goat anti-mouse secondary antibody, adding a target-specific imaging layer alongside core Cell Painting markers in the same well and imaging experiment.

The kit is useful when users want to enrich cell painting assays with a protein marker relevant to their biological question, such as a transcription factor, signaling protein, lineage marker, or disease-associated target. Compared with the PhenoVue Cell Painting Kits, this kit uses a staining configuration adapted for antibody-based multiplexing, with the PhenoVue 512 nucleic acid stain removed and dye concentrations adjusted to support the PhenoVue Fluor 400LS antibody detection channel.

The PhenoVue Cell Painting Target Add-on Kit (anti-mouse 400 LS) enables users to:

• Integrate a user-selected mouse primary antibody into cell painting workflows
• Add target-specific imaging information to multiparametric phenotypic profiling
• Support characterization of compound-induced cellular states
• Support phenotypic clustering and mechanism-of-action studies
• Monitor selected protein markers in the context of broader cellular morphology
• Adapt Cell Painting workflows to specific biological applications and cell models

Kit components and quantity:

• PhenoVue Hoechst 33342 Nuclear Stain, 1 vial (70 µL - 20 000X)
• PhenoVue Fluor 555 – WGA, 1 vial of 0.02mg
• PhenoVue Fluor 488 Concanavalin A, 1 vial of 1mg
• PhenoVue Fluor 568 Phalloidin, 1 vial of 0.4nmol
• PhenoVue 641 Mitochondrial Stain, 1 vial of 22µg
• PhenoVue Fluor 400LS - Goat anti-Mouse antibody Highly Cross-adsorbed,1 vial of 100µL (100X)
• PhenoVue dye diluent A (5X), 1 vial of 8mL (5X)