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HTRF Mouse IL-17 Detection Kit, 500 Assay Points

The HTRF mouse IL17 kit is designed for the quantification of mouse IL17 release in cell supernatant.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: 62MIL17PEG
Unit Size: 500 Assay Points
List price: USD 1,103.00
Your price:
USD 1,103.00
USD 1,103.00 /each
Part number: 62MIL17PEH
Unit Size: 10,000 Assay Points
List price: USD 10,290.00
Your price:
USD 0.00
USD 10,290.00 /each

Overview

IL17 is a pro-inflammatory cytokine induced by IL23 and produced by helper T cells. IL17 is part of response to fungi and bacteria, and promotes release of or pro-inflammatory cytokines and chemokines. Due to its role as a mediator of inflammatory responses, IL17 has been linked to allergic responses and autoimmune diseases like asthma, psoriasis, or IBD (inflammatory bowel disease). In immuno-oncology, IL17 is main cytokine released by differentiated TH17 cells.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma. When assaying, always follow recommended protocol and avoid highly haemolyzed samples.

Specifications

Assay Points
500
Assay Target Type
Kit
Assay Technology
HTRF
Brand
HTRF
Quantity
1
Shipping Conditions
Shipped in Dry Ice
Therapeutic Area
NASH/Fibrosis
Oncology & Inflammation
Unit Size
500 Assay Points

Video gallery

How it works

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

mouse-il17-kit

 

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

Assay details

Technical specifications of mouse IL17 kit
Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOd &LOQ (in Diluent) 4 pg/mL &16 pg/mL
Range 16 – 4,000 pg/mL
Time to result 1h at RT
Species Mouse only

 

Analytical performance

Intra and inter assay

Intra-assay (n=24)

Sample Mean [IL17] (pg/mL) CV
1 25 18%
2 1724 3%
3 3448 4%
  Mean CV 8%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample [IL17] (pg/mL) Mean (delta R) CV
1 41 273 5%
2 256 1678 5%
3 1600 9074 7%
    Mean CV 6%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

 

Resources

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

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