US
Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
HTRF Mouse IL-17 Detection Kit, 500 Assay Points
The HTRF mouse IL17 kit is designed for the quantification of mouse IL17 release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
IL17 is a pro-inflammatory cytokine induced by IL23 and produced by helper T cells. IL17 is part of response to fungi and bacteria, and promotes release of or pro-inflammatory cytokines and chemokines. Due to its role as a mediator of inflammatory responses, IL17 has been linked to allergic responses and autoimmune diseases like asthma, psoriasis, or IBD (inflammatory bowel disease). In immuno-oncology, IL17 is main cytokine released by differentiated TH17 cells.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma. When assaying, always follow recommended protocol and avoid highly haemolyzed samples.
Specifications
| Assay Points |
500
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Therapeutic Area |
NASH/Fibrosis
Oncology & Inflammation
|
| Unit Size |
500 Assay Points
|
Video gallery
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
Assay details
Technical specifications of mouse IL17 kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOd &LOQ (in Diluent) | 4 pg/mL &16 pg/mL |
| Range | 16 4,000 pg/mL |
| Time to result | 1h at RT |
| Species | Mouse only |
Analytical performance
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [IL17] (pg/mL) | CV |
|---|---|---|
| 1 | 25 | 18% |
| 2 | 1724 | 3% |
| 3 | 3448 | 4% |
| Mean CV | 8% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
| Sample | [IL17] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 41 | 273 | 5% |
| 2 | 256 | 1678 | 5% |
| 3 | 1600 | 9074 | 7% |
| Mean CV | 6% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Resources
Brochure
HTRF assays and reagents catalog
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
SDS, COAs, Manuals and more
Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.
Safety data sheet
-
LanguageEnglishCountryUnited States
-
LanguageFrenchCountryFrance
-
LanguageGermanCountryGermany
-
LanguageGreekCountryGreece
-
LanguageItalianCountryItaly
-
LanguageSpanishCountrySpain
-
LanguageEnglishCountryUnited Kingdom
+ Show more
Certificate of analysis
-
Lot number04FLot dateJanuary 31, 2025
-
Lot number04ELot dateJanuary 31, 2025
-
Lot number04HLot dateJanuary 31, 2025
+ Show more
IFUs, Manuals & more
-
Resource typeManualLanguageEnglishCountry-
How can we help you?
We are here to answer your questions.
