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AlphaScreen Nickel Chelate Donor Beads, 1 mg

Alpha Donor beads conjugated to nickel chelate. This bead can be used to capture His-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AS101D
Unit Size: 1 mg
List price: USD 757.38
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USD 757.38
USD 757.38 /each
Part number: AS101M
Unit Size: 5 mg
List price: USD 2,957.00
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USD 0.00
USD 2,957.00 /each
Part number: AS101R
Unit Size: 25 mg
List price: USD 13,750.00
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USD 0.00
USD 13,750.00 /each

Overview

Alpha Donor beads coated with nickel chelate. These beads can be used to capture His-tagged proteins and biomolecules, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:

  • Protein-protein interaction assays
  • Protein detection assays
  • Enzymatic assays

In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.

Specifications

Antibody Conjugates
Nickel chelate
Application
Protein-Protein Interaction
Automation Compatible
Yes
Bead Type or Material
Alpha Donor
Brand
AlphaLISA
Detection Method
Alpha
Format
Microplates
One Unit Contains
1.0 mg
Shipping Conditions
Shipped in Blue Ice
Unit Size
1 mg

Video gallery

Resources

1-3 of 3 Resources
Application Note Icon
Application Note
A fast and simple chemiluminescent assay for monitoring of DNA-protein interactions

Electrophoretic Mobility Shift Assay (EMSA) is a standard technique used to study protein-DNA interactions. It is a radioactive...

Guide Icon
Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...

Application Note Icon
Application Note
Biochemical binding ADCC assays utilizing AlphaLISA toolbox reagents for the characterization of hIgGs and FcγR1A

Fc Gamma Receptors (FcγRs) are critical for the function of therapeutic antibodies and mediate many effector functions.

Therapeutic...

SDS, COAs, Manuals and more

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Safety data sheet
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Certificate of analysis
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