This control lysate is intended for use with the AlphaLISA SureFire Ultra Human & Mouse Phospho-SYK (Tyr525/526) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U-937 were cells cultured in 10% FBS containing media. Cells were harvested and washed with HBSS + 0.1% BSA, adjusted to 2 x 106 cells/mL, then treated with 0.5 mM Pervanadate for 30 minutes. Cells were spun down at 300 RCF for 5 minutes and lysed in Lysis Buffer at 1 x 106 cells/mL.
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
SYK
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Unit Size |
1 vial
|
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