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AlphaLISA SureFire Ultra Human and Mouse Phospho-SYK (Tyr525/526) Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human & Mouse Phospho-SYK (Tyr525/526) assay. This control is already provided in the AlphaLISA SureFire Ultra Human & Mouse Phospho-SYK (Tyr525/526) assay kit but can also be ordered separately.

Feature Specification
Application Cell Signaling

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human & Mouse Phospho-SYK (Tyr525/526) assay. This control is already provided in the AlphaLISA SureFire Ultra Human & Mouse Phospho-SYK (Tyr525/526) assay kit but can also be ordered separately.

Product variant
Unit Size: 1 vial
Part #:
ALSU-PSYK-A-L
List price
USD 151.56
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra Human & Mouse Phospho-SYK (Tyr525/526) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U-937 were cells cultured in 10% FBS containing media. Cells were harvested and washed with HBSS + 0.1% BSA, adjusted to 2 x 106 cells/mL, then treated with 0.5 mM Pervanadate for 30 minutes. Cells were spun down at 300 RCF for 5 minutes and lysed in Lysis Buffer at 1 x 106 cells/mL.

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Modality
Alpha
Product Group
Control Lysate
Shipping Conditions
Shipped in Blue Ice
Target
SYK
Target Class
Phosphoproteins
Target Species
Human
Mouse
Technology
Alpha
Unit Size
1 vial

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