Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human & Mouse Phospho-SYK (Tyr525/526) assay. This control is already provided in the AlphaLISA SureFire Ultra Human & Mouse Phospho-SYK (Tyr525/526) assay kit but can also be ordered separately.
Feature | Specification |
---|---|
Application | Cell Signaling |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human & Mouse Phospho-SYK (Tyr525/526) assay. This control is already provided in the AlphaLISA SureFire Ultra Human & Mouse Phospho-SYK (Tyr525/526) assay kit but can also be ordered separately.
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human & Mouse Phospho-SYK (Tyr525/526) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U-937 were cells cultured in 10% FBS containing media. Cells were harvested and washed with HBSS + 0.1% BSA, adjusted to 2 x 106 cells/mL, then treated with 0.5 mM Pervanadate for 30 minutes. Cells were spun down at 300 RCF for 5 minutes and lysed in Lysis Buffer at 1 x 106 cells/mL.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
SYK
|
Target Class |
Phosphoproteins
|
Target Species |
Human
Mouse
|
Technology |
Alpha
|
Unit Size |
1 vial
|
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