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HTRF cAMP Gs HiRange Detection Kit, 1,000 Assay Points

The HTRF cAMP Gs HiRange Detection Kit is an immunoassay designed for the detection and quantitation of cAMP as a readout of Gs-coupled GPCR activity, in a homogeneous (no-wash steps, no separation steps) format.

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Feature	Specification
Application	Second Messenger Detection
Sample Volume	10 µL

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Product variants

Part #:

62AM6PEB

List price

USD 895.21

Your online price:

Part #:

62AM6PEC

List price

USD 5,574.70

Your online price:

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

Product information

Overview

Based on HTRF technology, the kit contains all the reagents needed to quantify cAMP, ensuring that you benefit from a complete tool for your needs.

Revvity's HTRF cAMP kits are best suited for measuring cAMP modulations in response to Gs-activation, leveraging an optimized combination of robustness and sensitivity

How it works

Assay principle

Revvity cAMP kits are based on a competitive format involving a specific antibody labeled with cryptate (donor) and cAMP coupled to d2 (acceptor). This enables the direct characterization of all types of compounds acting on Gs-coupled receptors in either adherent or suspension cells.

Assay protocol

The protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents.

Assay details

cAMP Gs hirange product specifications

If your experimental design uses membrane preparations, lipoparticles or purified receptors, or if your samples have large quantities of cAMP, the Gs HiRange kit will fit your needs!

The kit shows no ATP cross-reactivity up to 10mM, allowing the user to operate with large ranges of ATP and enabling membrane work.

Designed for optimal signal performance, cAMP - Gs HiRange offers a maximized signal-to-background score.

	S/B	IC20 nM	IC50 nM	IC80 nM
cAMP - Gs dynamic	>14	0.91	4.07	18.1
cAMP - Gs HiRange	>28	4.27	22.8	121

ATP cross-reactivity

Specifications

Other specifications

Application	Second Messenger Detection
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	10 µL
Shipping Conditions	Shipped Ambient
Target Class	GPCR
Technology	TR-FRET
Therapeutic Area	Cardiovascular Infectious Diseases Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size	1,000 assay points