PhenoVue Cell Painting Target Add-on Kit (anti-rabbit 400LS), 100 x 384

Cell painting is a phenotypic high-content imaging approach that combines multiplexed fluorescent staining, automated microscopy, and image analysis to characterize cellular responses to perturbagens such as compounds, drugs, or genes.

The PhenoVue Cell Painting Target Add-on Kit (anti-rabbit 400LS) extends cell painting workflows by enabling integration of a user-selected rabbit primary antibody into a multiparametric phenotypic profiling assay. The selected primary antibody is detected using the PhenoVue Fluor 400LS goat anti-rabbit secondary antibody, adding a target-specific imaging layer alongside core cell painting markers in the same well and imaging experiment.

The kit is useful when users want to enrich cell painting assays with a protein marker relevant to their biological question, such as a transcription factor, signaling protein, lineage marker, or disease-associated target. Compared with the PhenoVue Cell Painting Kits, this kit uses a staining configuration adapted for antibody-based multiplexing, with the PhenoVue 512 nucleic acid stain removed and dye concentrations adjusted to support the PhenoVue Fluor 400LS antibody detection channel.

The PhenoVue Cell Painting Target Add-on Kit (anti-rabbit 400LS) enables users to:

• Integrate a user-selected rabbit primary antibody into cell painting workflows
• Add target-specific imaging information to multiparametric phenotypic profiling
• Support characterization of compound-induced cellular states
• Support phenotypic clustering and mechanism-of-action studies
• Monitor selected protein markers in the context of broader cellular morphology
• Adapt Cell Painting workflows to specific biological applications and cell models

Kit components and quantity:

• PhenoVue Hoechst 33342 Nuclear Stain, 10 vials (70 µL - 20 000X)
• PhenoVue Fluor 555 – WGA, 10 vials of 0.2mg
• PhenoVue Fluor 488 Concanavalin A, 10 vials of 1mg
• PhenoVue Fluor 568 Phalloidin, 10 vials of 1nmol
• PhenoVue 641 Mitochondrial Stain, 20 vials of 50µg
• PhenoVue Fluor 400LS - Goat anti-Rabbit antibody Highly Cross-adsorbed, 10 vials of 1mg
• PhenoVue dye diluent A (5X), 10 vials of 80mL (5X)