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Cytotoxicity and Cell Proliferation Assays

Fast cytotoxicity and cell proliferation assays

Cytotoxicity assays and cell proliferation assays are widely used to measure cell growth, viability, and response in drug discovery.

Our ATPlite™ portfolio features patented ATP-based luminescence assays that provide a robust alternative to colorimetric, fluorometric, and radioisotopic methods for analyzing proliferation in mammalian cells.

These cytotoxicity and cell proliferation assays detect ATP using firefly luciferase, where light production is proportional to ATP concentration - a direct marker of metabolically active cells.

Choose the right format for your needs:
  • ATPlite 1glow-F/ATPlite 1glow-L - Single addition, frozen liquid or lyophilized, reagents with extended signal stability (>3 hours), wide dynamic range, and high-throughput workflow integration.
  • ATPlite 1step - Single addition assay for streamlined workflows
  • ATPlite - Extended signal stability for flexible timing
  • 3D applications - Both ATPlite™ 1step and ATPlite™ are available in specialized formats for 3D spheroids, microtissues, and organoids—providing more physiologically relevant results that better bridge the gap between traditional 2D cultures and in vivo models.

Explore our complete range of cytotoxicity and cell proliferation assays to find the perfect solution for your research needs.


For research use only. Not for use in diagnostic procedures.

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FAQs

  • What is a cytotoxicity assay?

    A cytotoxicity assay measures how toxic a substance is to living cells. It evaluates cell death, membrane damage, or loss of cellular function caused by drugs, chemicals, or treatments. Common applications include drug safety testing, cancer research, and toxicology studies.

  • What is a cell proliferation assay?

    A cell proliferation assay measures how fast cells grow and divide over time. It tracks cell multiplication under different conditions and is used for drug efficacy testing, cancer research, and studying cell cycle effects.

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