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miRNA-seq Analysis

Small RNA target discovery has never been so easy

There are different species of small non-coding RNAs (sncRNAs) that play critical roles in various regulatory processes such as transcription, post-transcription, and translation. Among those microRNAs (miRNAs) are the best characterized so far as gene expression regulators by post-transcriptional repression of their mRNA targets. Accumulating evidence highlights their crucial involvement in cancer development, underscoring their potential as biomarkers and therapeutic targets.

To simplify your cancer biomarker discovery, Revvity offers a complete small RNA-seq library preparation workflow that minimizes bias and human error while delivering exceptional miRNA discovery all in an automated gel-free workflow.


For research use only. Not for use in diagnostic procedures.

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MicroRNA sequencing (miRNA-seq) enables the discovery of novel miRNAs and other small noncoding RNAs, and the differential expression of all small RNAs in a sample.

Sample homogenization

Jumpstart your cancer research with a robust and powerful homogenizer designed to simplify sample preparation and prepare high quality lysates prior to nucleic acid extraction and miRNA workflows. 

 

Small RNA-seq extraction

To realize the full potential of miRNA as biomarkers, we offer a reliable workflow to extract efficiently circulating cell free RNA at high yield and purity.

 

Small RNA sizing and relative abundance analysis

The LabChip® small RNA assay provides a fast and easy way to separate and measure a variety of small RNA samples, ranging in size from 20 to 150 nucleotides (nt), including microRNA (miRNA), small interfering RNA (siRNA), piwi-interacting RNA (piRNA), RNA aptamers, transfer RNA (tRNA) and CRISPR guide RNA (gRNA). Performing small RNA sample analysis with the new small RNA assay saves time and money by automating steps for small RNA sizing and relative abundance.

 

Gel-free small RNA-seq library prep

The new NEXTFLEX® Small RNA-Seq Kit v4 allows preparation of small RNA libraries ready for sequencing on certain Illumina® platforms in approximately 6 hours with as little input as 1 ng of total RNA. Libraries prepared with this kit have a higher proportion of mapping reads and unique miRNAs than other workflows available in the market.

Automation of small RNA-seq workflows

Small RNA library preparation is one of the most difficult types of NGS library preparation, which can be simplified with automation to enable you to improve miRNA discovery.

The NEXTFLEX Small RNA-Seq Kit v4 is a gel-free, fully automatable protocol using an adapter dimer reduction approach. The advantage is that the protocol automated on the Sciclone® G3 NGS/NGSx and Zephyr® G3 NGS workstations enables high sample throughput, generating more libraries in less time while maintaining data quality.

 

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Please note that product labeling (such as kit insert, product label, and kit box) may be different compared to the company branding. Please contact your local representative for further details.