LabChip AAV Empty Full Characterization
Revvity’s complete LabChip® AAV empty/full characterization solution enables fast, high-throughput empty/full analysis with a low volume AAV input.
Improving your AAV empty full analysis with the LabChip GXII touch characterization system
Quantifying adeno-associated virus (AAV) titers and determining the ratio of AAV empty particles pose significant challenges in the development and manufacturing processes. Fast, easy, and low sample volume analysis is critical to ensure efficiency. Streamline your AAV characterization workflow. Traditional AAV characterization process involves using multiple separate machines for moderate AAV characterization. The use of so many instruments comes with several drawbacks:
- Complex Workflow: Managing many different instruments is labor-intensive and time-consuming. Researchers must coordinate their efforts across various devices, which can be challenging.
- Increased Risk and Error: Each additional instrument introduces the risk of errors, calibration discrepancies, and technical glitches. The more steps involved, the greater the chance of inaccuracies creeping into the results.
By optimizing your AAV characterization workflow with the LabChip™ GXII Touch™ characterization system, you can achieve more reliable results, reduce workload, and draw meaningful conclusions about AAV product quality.
Speed of analysis, throughput, and low input amounts are additional advantages the LabChip GXII Touch characterization system offers over slower and more costly traditional AAV analysis methods including transmission microscopy (TEM), analytical ultracentrifugation (AUC), charge-based chromatographic techniques, and UV Absorbance spectroscopy.
Get the complete AAV empty/full ratio in minutes.
The LabChip GXII Touch™ Characterization System is a microfluidic capillary electrophoresis-based solution which enables rapid, high-throughput empty/full analysis with a low volume AAV input. The LabChip AAV assay analyzes both ssDNA and proteins to quickly assess the concentration, purity, and empty/full ratio of the AAV capsid. Microliter sized samples are collected and analyzed in microfluidic channels measuring both proteins and nucleic acids in ~ 1 minute per sample. The high-throughput capability of the LabChip GXII Touch system enables the determination of product purity, genomic integrity, and empty/full analysis for up to 96 total samples per run.
Get the complete empty full picture in minutes.
The LabChip AAV assay is an all-in-one solution that analyzes both DNA and proteins to quickly assess the concentration, purity, VP stoichiometry, and empty/full ratio of the AAV capsid in purified samples.
For research use only. Not for use in diagnostic procedures.
AAV Samples (only 24 ul/sample needed)
LabChip® AAV DNA Analysis + LabChip AAV Protein Analysis =
- Reliable prediction of empty/full
- Partials estimation
- Purity assessment of viral proteins
- Genomic integrity of genomic insert (ssDNA)
- Purity of genomic insert
- Sizing of genomic insert
AAV empty full analysis data
Example viral protein egrams
Example DNA egrams
Revvity’s LabChip AAV empty full solution
To meet individual laboratories’ needs, the LabChip AAV Empty/Full Solution is provided in bundles.
| AAV Bundles |
|---|
| LabChip® AAV Empty/Full Reagents bundle + LabChip® AAV Empty/Full Chip bundle |
Or the components can be ordered separately:
| AAV DNA Analysis | AAV Protein Analysis |
|---|---|
|
LabChip® AAV DNA Analysis Kit LabChip® AAV DNA Chip LabChip® AAV8 standard |
LabChip® AAV Pico Protein Analysis kit LabChip® AAV Protein Analysis Chip LabChip® AAV8 standard |
Improve the efficiency of your protein development & manufacturing workflows
The LabChip GXII Touch characterization system provides a complete solution for reproducible quantitation, molecular weight sizing and percent purity analysis of protein samples. Learn how it can improve the efficiency of your protein characterization workflows.
Kit Specs
| Requirement | Specification |
|---|---|
|
Minimum Sample Volume Input Minimum AAV Sample Concentration AAV Sample Buffer Empty:Full Value Linearity Protein Assay Sensitivity ssDNA Assay Sensitivity Samples per chip preparation |
10 μL (5 uL + 5 uL) 1E12 VP/mL (or 1E12GC/mL for high population of empty AAV) PBS + (0.01% Tween 20 or 0.001% Pluronic) 0.95 5 ng/μL 250 pg/μL 24 |
Why are AAV particles being investigated for therapeutic use in gene therapy solutions?
Adeno-associated virus (AAV) particles are intensively being investigated for therapeutic use in gene therapy due to low immunogenicity and ability for long-term gene expression in vivo.
What is an AAV viral vector?
The AAV viral vector is comprised of a protein capsid with encapsulated ssDNA. The protein capsid is composed of three capsid proteins VP1, VP2 and VP3 with theoretical expected ratio of 1:1:10 respectively. The proteins are packaged into a stable icosahedral structure with diameter ~25 nm.
The encapsulated ssDNA is typically up to ~4.8 kb and are the therapeutic payloads that are critical to treating various diseases. During development, testing strategies for the viral protein stoichiometry, capsid product purity, and ssDNA loading efficacy are used to monitor and assess AAV efficacy.
How is the AAV loading efficiency determined?
The loading efficacy is observed as the percentage of capsids containing ssDNA (full capsids).
What are ineffective AAV capsids?
Ineffective capsids may contain no ssDNA (empty capsids) or DNA that is lower molecular weight (partial filled capsids).
