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CRISPR Technologies

Make targeted, specific edits to any gene

Gene editing refers to the process of changing regions of cellular DNA. The most common gene editing techniques involve inactivating a gene’s function (knockout), introducing or correcting a SNP mutation, or adding a reporter tag to an endogenous gene (knock-in). These changes are permanent and heritable, resulting in a newly engineered cell line.

With our Revvity Dharmacon™ Edit-R™ CRISPR-Cas9 reagents, researchers can make targeted, specific edits to any gene in practically any cell type. Additionally, a wide selection of pre-made edited cell lines and expert genome engineering services are available.
 

For research use only. Not for use in diagnostic procedures. 

CRISPR knockout

Algorithm designed CRISPR guide RNAs for highly accurate and efficient functional gene knockout.

Our guide RNAs are designed using a validated algorithm to achieve functional gene knockout with high specificity. By assessing phenotypes for thousands of designs, then validating our design rules in multiple assay systems, we have established standards for determining optimal knockout target sites.

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CRISPR knock-in

CRISPR guide RNAs program Cas9 nuclease to cut genomic DNA at a specific location. Once the double-strand break (DSB) occurs, the mammalian cell utilizes endogenous mechanisms to repair the DSB. In the presence of a donor DNA, either a ssDNA oligo or a plasmid donor, the DSB can be repaired precisely using HDR resulting in a desired genomic alteration (insertion, removal, or replacement). 

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CRISPR interference

CRISPR interference (CRISPRi) allows researchers to down-regulate specific gene function by blocking transcription, without editing the DNA.  

The Revvity Dharmacon CRISPRmod™ CRISPRi system requires two components to operate: a gene-specific CRISPRi guide RNA and a catalytically deactivated Cas9 (dCas9) fused to transcriptional repressor domains (SALL1 and SDS3). Our CRISPRi reagents provide a straightforward, efficient set of tools to study a gene’s function via transcriptional repression. 

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CRISPR activation

CRISPR activation (CRISPRa) allows researchers to up-regulate specific gene function by activating transcription, without editing the DNA.

The Revvity Dharmacon CRISPRmod CRISPRa system requires two components to operate: a CRISPRa guide RNA and a catalytically deactivated Cas9 (dCas9) fused to transcriptional activators (VPR). Our CRISPRa reagents provide a straightforward, efficient set of tools to study a gene’s function by overexpression in its native context. 

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Pin-point™ base editing reagents

Revvity's Pin-point base editing technology is a three-component system featuring a nickase Cas9 which recruits the effector deaminase through fusion to an aptamer-binding protein. The aptamer is linked to the guide RNA associated with the nuclease in an extended, chimeric RNA scaffold. The modular nature of effector recruitment means that nCas9 can be used for the knockout of one or more gene targets while simultaneously inserting a transgene. 

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Gene editing libraries

Achieve high functionality and superior specificity in your pooled and arrayed screens using algorithm-optimized guide RNA designs. Dharmacon synthetic sgRNA arrayed, crRNA arrayed, and lentiviral sgRNA pooled or lentiviral sgRNA arrayed for high-throughput gene editing studies.

Investigate entire gene families or biological pathways with our broad suite of custom and pre-defined libraries of Dharmacon Edit-R CRISPR-Cas9 reagents. 

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Please note that product labeling (such as kit insert, product label, and kit box) may be different compared to the company branding. Please contact your local representative for further details.