Automated Methods: DNA Library Preparation Kits
DNA library prep, a crucial step in NGS workflows
Careful DNA library preparation is paramount to achieving accurate results in next-generation sequencing (NGS). It involves fragmenting DNA molecules, attaching sequencing adapters, and amplifying the resulting fragments to create a library suitable for sequencing.
Revvity automates a complete line of DNA library preparation kits on our liquid handling portfolio to meet your sequencing needs.
Our vendor-qualified methods eliminate Library preparation bottlenecks. Methods for fragmenting DNA include chemical and enzymatic fragmentations.
Modernizing DNA library preparation: an automated workflow for translational research and clinical genomics
Poster by Revvity and Watchmaker Genomics presented at ABRF in 2023
Convert your DNA into sequencing-ready fragments in just a few steps.
Modernize your DNA library preparation with an automated workflow on Revvity liquid handlers with one of the currently available vendor-qualified methods below.
Revvity provides a complete portfolio of NGS library preparation kits and barcodes designed to increase the flexibility and speed of library prep for customers utilizing Illumina® and Element Biosciences® sequencing platforms.
We continuously add new qualified methods, please contact us if you do not see the one you need.
For research use only. Not for use in diagnostic procedures.
DNA Library Preparation Kits automated on Revvity liquid handlers
| Vendor | Library Prep Kit | Sciclone™ G3 NGSx | Sciclone™ G3 NGSx iQ™ | Zephyr™ G3 NGS | Fontus™ NGS 96 | BioQule™ |
|---|---|---|---|---|---|---|
| 10x Genomics® | Genomic Preparation | X | ||||
| Agilent® | SureSelect XT HS2 DNA | X* | X* | |||
| ArcherDx® | Universal DNA Reagent Kit v2 | X | ||||
| CareDx® | AlloSeq TZ | X | ||||
| Celemics® | Whole Exome Sequencing (WES), Library Preparation, Target Enrichment | X | ||||
| Claret Bioscience | ClaretBio® SRSLY® PicoPlus Kit | X | ||||
| IDT® | xGen DNA Library Prep Kit EZ | X | X | |||
| IDT® | xGen cfDNA & FFPE DNA Library Prep MC v2 Kit | X | ||||
| IDT® | xGen™ Pre-Hybridization Capture Normalase™ Module with xGen DNA Library Prep Kit EZ | In Dev | ||||
| IDT® | Accel-NGS® 2S Plus DNA Library Kit | X | ||||
| IDT® | xGen Amplicon Panels | X | X | |||
| Illumina® | Illumina® Complete Long Reads with Enrichment | X | ||||
| Illumina® | Illumina® DNA Prep with Exome 2.0 Plus Enrichment | X | X | |||
| Illumina® | Illumina® DNA PCR-Free Library Prep Tagmentation | X | X | X* | X | |
| Illumina® | Illumina® DNA Prep (previously called Nextera DNA Flex) | X | X | X | X | |
| Illumina® | Illumina® DNA Prep with Enrichment | X | X | |||
| Illumina® | Illumina® DNA Prep with Exome 2.5 Enrichment | X | X | |||
| Illumina® | Nextera® XT DNA Kit | X | X | X | ||
| Illumina® | TruSeq® DNA PCR-Free Kit | X | X | |||
| Illumina® | TruSeq® Nano DNA Kit | X | ||||
| New England Biolabs® / NEB® | NEBNext® dsDNA Fragmentase® Kit | X | ||||
| NEB® | NEBNext® Ultra™ DNA Kit | X | X | |||
| NEB® | NEBNext® Ultra™ II DNA Library Prep for Illumina® Instruments | X | X | |||
| NuGen® / Tecan® | Celero™ DNA-Seq Library Prep Kit | X | ||||
| Oxford Nanopore Technologies (ONT) | Ligation Sequencing Kit V14 Protocol | In Dev | In Dev | X | ||
| PacBio® | HiFi SMRTbell® Libraries with SMRTbell Express Template Prep Kit 2.0 | X | X | |||
| PacBio® | HiFi SMRTbell® Libraries with SMRTbell Prep Kit 3.0 | [X] | ||||
| PacBio® | Multiplexed Microbial Library Prep with SMRTbell® Express Template Prep Kit 2.0 | X | X | |||
| QIAGEN® | QIAGEN® Targeted DNA v3 Panel | X | X | |||
| QIAGEN® | QIAseq® FX DNA Library Kit | X | ||||
| QIAGEN® | QIAseq® Normalizer Kit | In Dev | ||||
| Revvity | NEXTFLEX™ Cell Free DNA-Seq Kit 2.0 | X | X | X | ||
| Revvity | NEXTFLEX™ Rapid DNA-Seq Kit 2.0 | X | X | X | ||
| Revvity | NEXTFLEX™ Rapid XP v2 DNA-Seq Kit | [X] | X | X | X** | |
| Revvity | PG-Seq™ Rapid Kit v2 | X | ||||
| Roche® | Kapa EvoPlus Kit | X | ||||
| Roche® | DNA HyperPlus™ Kit | X | X | X | ||
| Roche® | DNA HyperPrep™ Kit | X | X | X | X | |
| seqWell® | plexWell™ 96 | In Dev | ||||
| Thermo Fisher® | Ion Express® Plus gDNA Fragment Library Prep Kit | X | X | |||
| Thermo Fisher® | Ion Torrent™ Ion Express® Fragment Library Prep Kit | X | X | |||
| TwinStrand® Bioscience | TwinStrand® Duplex Sequencing: Pre-PCR (Mechanical Shearing) | X | ||||
| TwinStrand® Bioscience | TwinStrand® Duplex Sequencing: Pre-PCR (Enzymatic Shearing) | X | ||||
| TwinStrand® Bioscience | TwinStrand® Duplex Sequencing: Post-PCR | X | ||||
| Twist® | Library Preparation Kit | X | X | |||
| Watchmaker Genomics | DNA Library Prep | X | ||||
| Watchmaker Genomics | DNA Library Prep with Fragmentation | X |
* Chemistry tested at customer site
** Water tested
In Dev = in development
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