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PhenoVue ROS-570, Total Oxidative Stress Indicator

PhenoVue™ ROS-570, Total Oxidative Stress Indicator is a cell permeable fluorescent reactive oxygen species indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue ROS-570, Total Oxidative Stress Indicator exhibits orange fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 574 nm and a maximum emission wavelength of 588 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

Part number: CP15O
Quantity: 1 x 1 mg
List price: USD 592.70
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USD 592.70
USD 592.70 /each

Overview

Reactive oxygen species (ROS) are naturally produced by all living organisms and participate in cell signaling, gene expression and homeostasis. Increasing levels of ROS is associated with aging, as well as with various diseases such as diabetes, pulmonary disorders, inflammatory diseases, and cancer. ROS are also produced by exogenous sources such as UV or heat. Ultimately elevated ROS concentration, which is known as oxidative stress, results in impairment of membrane lipids, proteins, and nucleic acids.

ROS are reactive molecules which contain oxygen and encompass various entities such as superoxide anion (O2-), hydrogen peroxide (H2O2), hydroxyl radical (•OH), hypochlorous acid (HOCl), peroxynitrite anion (ONOO-), and nitric oxide (NO).

PhenoVue ROS Total Oxidative Stress Indicators, encompassing ROS-490, ROS-570 and ROS-670, enable a myriad of applications as well as multiplexing capability to unravel ROS related biological effects.

PhenoVue ROS-570, Total Oxidative Stress Indicator is a nonfluorescent cell-permeable probe which emits bright orange fluorescence upon reaction with hydroxyl radical, hydrogen peroxide and hypochlorite anion.

Features:

  • Numbers of Vials Per Unit: 1
  • Quantity or Volume Per Vial: 1 mg (1 mg-1.365 µmoles)
  • Form: Solid
  • Storage: -16 °C
  • Recommended Working Concentration: 1-10 µM
  • Maximum Excitation Wavelength: 574 nm
  • Maximum Emission Wavelength: 588 nm
  • Common Filter Set: Cy3/TRITC
  • Live Cell Staining: Yes
  • Fixed Cell Staining: No

Equivalent Number of Microplates:

  • 4-14 x 96-well microplates
  • 4-14 x 384-well microplates
  • 16-22 x 1536-well microplates

Specifications

Color
Orange
Application
High Content Imaging
Microscopy
Brand
PhenoVue
Detection Modality
Fluorescence
Filter
Cy3/TRITC
Quantity
1 x 1 mg
Sample Type
Live samples only
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
-16 °C or below, protected from light
Type
Individual Reagent

Spectra Viewer

Resources

1-5 of 5 Resources
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PhenoVue Cellular Imaging Reagents Brochure
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PhenoVue Cellular Imaging Reagents Flyer

This flyer describes Revvity's PhenoVue cellular imaging reagents.

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PhenoVue Reagents & Imaging Microplates Product List
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Product Info
Phenovue ROS, Total Oxidative Stress Indicators Product Sheet

This is a product information sheet for Phenovue ROS, Total Oxidative Stress Indicators

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Product Info
Phenovue ROS, Total Oxidative Stress Indicators Product Sheet

This is a product information sheet for Phenovue ROS, Total Oxidative Stress Indicators

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