Oxygen molecules support the intracellular energy production and are used by several biochemical processes. Hypoxia, or low oxygen levels, is therefore a significant stress that often impairs aerobic species' ability to survive and is a key component of the pathological conditions such as inflammation, cardiovascular diseases, and cancer. As such, understanding molecular mechanisms underlying hypoxia inducing conditions is crucial. For example, low oxygen level results in the upregulation of Hypoxia inducible factors (HIFs) which in turn regulate the expression of genes involved in cell survival, angiogenesis, glycolysis, and invasion/metastasis.

PhenoVue HypoxiTRAK is a far-red fluorescent molecule that readily enters live cells. Its spectral characteristics are shared with DRAQ5™.

HypoxiTRAK is converted to a metabolite, under hypoxic conditions, which persists in cells for several days allowing a simple determination of the extent of hypoxia, without antibody staining. It is non-toxic and suited to continuous exposure that allows the generation of hypoxia dependent changes in cell biology. The HypoxiTRAK-metabolite is designed to halt cell cycle progression, preserving the absolute number of hypoxia-experiencing cells. Cells sensitive to cycle arrest induced by the HypoxiTRAK-metabolite may undergo cell death. The non-viable cell fraction is a simple surrogate readout of the hypoxia experience of cells.

Features:

• Numbers of Vials Per Unit: 1
• Quantity or Volume Per Vial: 500 µL (10 µM - 5 nmoles)
• Form: Liquid
• Storage: 2-8 °C (Do not freeze)
• Recommended Working Concentration: 50 nM
• Maximum Excitation Wavelength: 646 nm
• Maximum Emission Wavelength: 697 nm
• Common Filter Set: Cy5
• Live Cell Staining: Yes
• Fixed Cell Staining: No

Equivalent Number of Microplates:

• 3-10 x 96-well microplates
• 3-10 x 384-well microplates
• 5-16 x 1536-well microplates