PhenoVue Fluor 647 - Live Cell Tubulin Stain

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PhenoVue Fluor 647 - Live Cell Tubulin Stain

SDS

PhenoVue Fluor 647 Live Cell Tubulin Stain

HeLa cells stained with PhenoVue 551 Mitochondrial Stain

U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.

U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)

CRISPR Knockout Aurora Kinase A. U2OS CAG-Cas9 stable cell line. Stained with PhenoVue 512 Nucleic Acid Stain. Imaged on Opera Phenix Plus system, 40x water immersion objective.

U2OS live cells stained with PhenoVue 551 Mitochondrial Stain (mitochondria, orange). Imaged on Opera Phenix Plus.

U2OS cells stained with PhenoVue Fluor 647 Live Cell Tubulin Stain & PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus, 63X water objective confocal.

U2OS cells stained with PhenoVue Fluor 568 - Phalloidin, PhenoVue Fluor 555 - WGA, PhenoVue Fluor 488 - Concanavalin A, PhenoVue Fluor 641 Mitochondrial Stain, and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus, 63X Water Confocal.

Composite images of HeLa cells (treated with fembendazole) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

U2OS Cells Stained with PhenoVue Fluor 555 WGA & PhenoVue Fluor 568 - Phalloidin. Imaged on Opera Phenix Plus, confocal, 40x water immersion objective.

Opera Phenix Plus High-Content Screening system for high-throughput high-content assays.

Opera Phenix Plus High-Content Screening system for fast response assays, phenotypic screening, and assays using complex cell models.

Picture of HeLa cells treated with etoposide

Composite images of HeLa cells (treated with berberine) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

CaFlux Assay iPSC-dervied neurons (nexel) stained with Cal-520 on Opera Phenix Plus (20xW).

U2OS live cells stained with PhenoVue Fluor 647 Live Cell Tubulin Stain (tubulin, red) & PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Opera Phenix Plus.

Composite images of HeLa cells (treated with tetrandine) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

Zebrafish 72hpf – Neutrophils. Lateral positioning on agarose. Imaged by PreciScan on Phenix Plus, 20xAir, Widefield : Brighfield / Ds Red (MIP). Image provided by Institut Pasteur Paris (PBI).

CRISPR Knockout Aurora Kinase A. U2OS CAG-Cas9 stable cell line. Stained with PhenoVue Hoechst 33342 Nuclear Stain. Imaged on Opera Phenix Plus system, 40x water immersion objective.

CRISPR Knockout Aurora Kinase A. U2OS CAG-Cas9 stable cell line. Stained with PhenoVue Fluor 488 Concavalin A. Imaged on Opera Phenix Plus system, 40x water immersion objective.

C2C12 cells stained with PhenoVue Fluor 647 Live Cell Tubulin Stain & PhenoVue Hoechst 33342 Nuclear Stain. Imaged on Opera Phenix Plus.

Composite images of HeLa cells (treated with CA74) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

COS7 cells stained with PhenoVue Fluor 647 Live Cell Tubulin Stain & PhenoVue Hoechst 33342 Nuclear Stain. Imaged on Opera Phenix Plus.

Composite images of HeLa cells (treated with cytochalasin) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

U2OS cells stained with PhenoVue 561 Mitochondrial Stain (Orange), PhenoVue Fluor 647 Live Cell Tubulin Stain (Red) and PhenoVue Hoechst 33342 (Blue). Imaged on Opera Phenix Plus.

Opera Phenix Plus High-Content Screening system with liquid handling module and fast imaging frame rate for fast-response assays.

U2OS Cells Stained with PhenoVue Fluor 641 Mitochondrial Stain. Imaged on Opera Phenix Plus, confocal, 40x water immersion objective.

MRC5 live cells stained with PhenoVue Fluor 647 Live Cell Tubulin Stain (tubulin, red) & Phenovue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Opera Phenix Plus.

U2OS Cells Stained with PhenoVue Fluor 488 Concavalin A. Imaged on Opera Phenix Plus, confocal, 40x water immersion objective.

Composite image of HeLa cells (untreated) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

U2OS Cells Stained with PhenoVue 512 Nucleic Acid Stain. Imaged on Opera Phenix Plus, confocal, 40x water immersion objective.

U2OS Cells Stained with PhenoVue Hoechst 33342 Nuclear Stain. Imaged on Opera Phenix Plus, confocal, 40x water immersion objective.

CRISPR Knockout Aurora Kinase A. U2OS CAG-Cas9 stable cell line. Stained with PhenoVue Fluor 555-WGA & PhenoVue Fluor 568-Phalloidin. Imaged on Opera Phenix Plus system, 40x water immersion objective.

CRISPR Knockout Aurora Kinase A. U2OS CAG-Cas9 stable cell line. Stained with PhenoVue Fluor 641 Mitochondrial stain. Imaged on Opera Phenix Plus system, 40x water immersion objective.

PhenoVue™ Fluor 647 – Live Cell Tubulin Stain, is a cell permeable fluorogenic dye which specifically binds to polymerized tubulin and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Fluor 647 – Live Cell Tubulin Stain can be used to visualize microtubules in live cells, exhibiting far red fluorescence, and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 650 nm and a maximum emission wavelength of 670 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

Product information

For research use only, not for use in diagnostic procedures.

SDS

Product information

Overview

PhenoVue Fluor 647 Live Cell Tubulin Stain is a cell permeable fluorogenic dye which specifically binds to polymerized tubulin and can be used for microtubule cytoskeleton imaging in live cells.

PhenoVue Fluor 647 Live Cell Tubulin is a no-wash, sensitive, bright and photostable stain, which can be multiplexed with other blue, green, or orange cell permeable stains, such as PhenoVue Nuclear, Lysosomal or Mitochondrial stains.

Taxane derivatives, such as Paclitaxel and Docetaxel, which are commonly used in chemotherapy, inhibit microtubules depolymerization and cause cell cycle arrest. Like other tubulin stains derived from taxane, cytotoxicity and cell cycle impairment can be observed with long exposure time (>24h) at high concentration. This can be significantly limited at concentrations between 50 and 300 nM, while maintaining high brightness and image quality.

Depending on the cellular model, intracellular retention of PhenoVue Fluor 647 Live Cell Tubulin Stain can be further improved in the presence of efflux pump inhibitor, such as PhenoVue Probenecid, Ready to Use Solution.

Features:

Numbers of Vials Per Unit: 2
Quantity or Volume Per Vial: 50 nmoles
Form: Dessicated
Storage: -16 °C
Recommended Working Concentration: 300 nM
Maximum Excitation Wavelength: 650 nm
Maximum Emission Wavelength: 670 nm
Common Filter Set: Cy5
Live Cell Staining: Yes
Fixed Cell Staining: No
Equivalent Number of Microplates:
- 5 to 17 x 96-well microplates
- 5 to 17 x 384-well microplates
- 9 to 27 x 1536-well microplates

Specifications

Physical Specifications

Color	Red

Other Specifications

Application	High Content Screening Imaging Microscopy
Brand	PhenoVue^™
Detection Method	Fluorescence
Filter	Cy5
Organelle and Cell Compartment	Tubulin
Product Compatibility	Live samples only
Shipping Conditions	Shipped in Dry Ice
Type	Individual Reagent
Unit Size	2 vials