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PhenoVue Fluor 400LS - Phalloidin

PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus. View All
PhenoVue Fluor 400LS - Phalloidin
PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.
PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.
PhenoVue Fluor 400LS - Phalloidin
PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.
PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.

PhenoVue Fluor 400LS - Phalloidin, part of our portfolio of cellular imaging reagents, is a long Stokes shift fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

This dye exhibits bright orange fluorescence and can be easily multiplexed for imaging and high-content screening applications. Additionally, it is validated on Revvity' HCS instruments such as Operetta® CLS™ and Opera Phenix® Plus.

PhenoVue Fluor 400LS - Phalloidin has a maximum excitation wavelength of 395 nm and a maximum emission wavelength of 585 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

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Feature Specification
Color Orange
Filter Ex: 375-440 nm, Em: 550-650 nm
Fluorophore PhenoVue™ Fluor 400LS
Organelle and Cell Compartment Actin
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PhenoVue Fluor 400LS - Phalloidin, part of our portfolio of cellular imaging reagents, is a long Stokes shift fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

This dye exhibits bright orange fluorescence and can be easily multiplexed for imaging and high-content screening applications. Additionally, it is validated on Revvity' HCS instruments such as Operetta® CLS™ and Opera Phenix® Plus.

PhenoVue Fluor 400LS - Phalloidin has a maximum excitation wavelength of 395 nm and a maximum emission wavelength of 585 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
View product information
Product Variant
Quantity: 1 x 300 units
Part #:
CP24001
List Price
USD 753.59
PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.
PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.
PhenoVue Fluor 400LS - Phalloidin
PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.
PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.
PhenoVue Fluor 400LS - Phalloidin
PhenoVue Fluor 400LS - Phalloidin
PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.
PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.

Product information

Overview

Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.

Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.

PhenoVue Fluor 400LS - Phalloidin can be used to visualize actin filaments in indirect immunofluorescence, immunohistochemistry as well as high-content analysis and screening applications. ​​Its unique photophysical property enables extended multiplexing capability by adding a fifth color while ensuring no spectral overlap, high brightness, as well as photostability.

Features

  • Number of Vials Per Unit: 1
  • Quantity or Volume Per Vial: 300 units (10 nmoles; 12 µg)
  • Form: Desiccated
  • Storage: -20 °C
  • Recommended Working Concentration: 165 nM (0.20 µg/mL)
  • Maximum Excitation Wavelength: 395 nm
  • Maximum Emission Wavelength: 585 nm
  • Live Cell Staining: No
  • Fixed Cell Staining: Yes
  • Equivalent Number of Microplates:
    • 2 - 6 x 96-well microplates
    • 2 - 6 x 384-well microplates
    • 3 - 10 x 1536-well microplates

Specifications

Color
Orange
Form
Desiccated
Maximum Emission Wavelength (Emmax)
585 nm
Maximum Excitation Wavelength (Exmax)
395 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
Ex: 375-440 nm, Em: 550-650 nm
Fluorophore
PhenoVue™ Fluor 400LS
Organelle and Cell Compartment
Actin
Quantity
1 x 300 units
Sample Type
Fixed samples only
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
-16 °C or below, protected from light
Type
Individual Reagent

Image gallery

PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.
PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.
PhenoVue Fluor 400LS - Phalloidin
PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.
PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.
PhenoVue Fluor 400LS - Phalloidin
PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.
PhenoVue Fluor 400LS - Phalloidin
U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)
U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.

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Resources

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Technical Note
Expanding imaging multiplexing capabilities with PhenoVue Fluor 400LS - Phalloidin

This technical note offers tips and best practices for using PhenoVue® Fluor 400LS Phalloidin stain to optimize fluorescent signal...

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PhenoVue Cellular Imaging Reagents Brochure
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PhenoVue Cellular Imaging Reagents Flyer

This flyer describes Revvity's PhenoVue cellular imaging reagents.

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PhenoVue Fluor - Phalloidin Conjugates Product Information Sheet

This is a product information sheet for PhenoVue Fluor - Phalloidin Conjugates

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PhenoVue Reagents & Imaging Microplates Product List
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