PhenoVue DNA Damage and Apoptosis Staining Kit - 1 x 384 (gH2AX, cleaved PARP)

DNA is continuously exposed to insults that can result in strand breaks, replication stress, or chemical modifications. If left unrepaired, these lesions can compromise genome integrity and may lead to genomic instability, senescence, or cell death. Cells counteract these threats by activating the DNA Damage Response (DDR), a signaling network that detects lesions, promotes repair, and contributes to cell fate decisions.

Phosphorylation of histone H2AX at serine 139 (γ-H2AX) is a widely used marker of DNA double-strand breaks, while cleaved PARP1 serves as a marker of apoptotic pathway activation. Combined analysis of both markers provides complementary information on DNA damage signaling and apoptosis within fixed-cell imaging workflows.

The PhenoVue DNA Damage and Apoptosis Staining Kit combines PhenoVue Hoechst 33342 Nuclear Stain with antibodies targeting γ-H2AX and cleaved PARP1, compatible with high-content imaging systems for characterization of compounds or conditions that induce DNA damage or trigger apoptosis.

For enhanced flexibility, up to two additional biological markers can be included using the PhenoVue Fluor 555 and 400LS channels, allowing for deeper insights into DNA damage response (DDR) mechanisms.

Kit components and quantity:

• PhenoVue Hoechst 33342 Nuclear Stain, 1 vial of 70 µL (500X)
• PhenoVue anti-Phospho H2AX (S139) – mouse IgG1 Antibody (100X), 1 vial of 100 µL (100X)
• PhenoVue anti-cleaved PARP1 Antibody (Rabbit), 1 vial of 100 µL (100X)
• PhenoVue Fluor 488 - Goat anti-Mouse antibody Highly Cross-Adsorbed (100X), 1 vial of 100 µL (100X)
• PhenoVue Fluor 647 - Goat anti-Rabbit antibody Highly Cross-Adsorbed (100X) , 1 vial of 100 µL (100X)
• PhenoVue Paraformaldehyde, 4% Solution, 1 vial of 25 mL (1X)
• PhenoVue Permeabilization, 0.5% Triton X-100 Solution, 1 vial of 25 mL (1X)
• PhenoVue dye diluent A (5X), 1 vial of 8 mL (5X)