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PhenoVue 505 Live Cell Caspase-3/7 Activity Stain
PhenoVue 505 Live Cell Caspase-3/7 Activity Stain
PhenoVue™ 505 live cell caspase-3/7 activity stain is a no wash, cell permeable fluorogenic dye which is specifically cleaved by activated pro apoptotic caspases 3 and 7 in living cells, leading to fluorescence increase.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue 505 live cell caspase-3/7 activity stain, has a maximum excitation wavelength of 505 nm and a maximum emission wavelength of 530 nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
| Feature | Specification |
|---|---|
| Cell Function Indicator | Apoptosis indicator |
| Color | Green |
| Filter | FITC |
PhenoVue™ 505 live cell caspase-3/7 activity stain is a no wash, cell permeable fluorogenic dye which is specifically cleaved by activated pro apoptotic caspases 3 and 7 in living cells, leading to fluorescence increase.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue 505 live cell caspase-3/7 activity stain, has a maximum excitation wavelength of 505 nm and a maximum emission wavelength of 530 nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
PhenoVue 505 Live Cell Caspase-3/7 Activity Stain
PhenoVue 505 Live Cell Caspase-3/7 Activity Stain
Product information
Overview
PhenoVue 505 live cell caspase-3/7 activity stain is a no wash, cell permeable fluorogenic dye which is specifically cleaved by activated pro apoptotic caspases 3 and 7 in living cells, leading to fluorescence increase.
Sensitive, rapid and photostable, PhenoVue 505 live cell caspase-3/7 activity stain exhibits green emission and can be multiplexed with blue, orange and red colors.
PhenoVue 505 live cell caspase-3/7 activity stain uses a DEVD substrate conjugated with a non-fluorescent cell permeable DNA binding dye which becomes fluorescent when cells undergo apoptosis.
Additional product information
Features
| Numbers of vials per unit | 1 |
|---|---|
| Quantity or volume per unit | 100 µL (1 mM DMSO) |
| Form | Liquid |
| Storage | -16 °C |
| Recommended working concentration | 5 µM |
| Maximum excitation wavelength | 505 nm |
| Maximum emission wavelength | 530 nm |
| Common filter set | FITC |
| Live cell staining | Yes |
| Fixed cell staining | No |
| Equivalent number of microplates | 0.5-2 x 96-well microplates 0.5-2 x 384-well microplates 1-3 x 1536-well microplates |
Specifications
| Color |
Green
|
|---|---|
| Form |
Solution in DMSO
|
| Maximum Emission Wavelength (Emmax) |
530 nm
|
| Maximum Excitation Wavelength (Exmax) |
505 nm
|
| Application |
High Content Imaging
Microscopy
|
|---|---|
| Brand |
PhenoVue™
|
| Cell Function Indicator |
Apoptosis indicator
|
| Detection Modality |
Fluorescence
|
| Filter |
FITC
|
| Quantity |
1 x 100 µL
|
| Sample Type |
Live samples only
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Storage Conditions |
-16 °C or below, protected from light
|
| Type |
Individual Reagent
|
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Resources
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This flyer describes Revvity's PhenoVue cellular imaging reagents.
This is a product information sheet for PhenoVue live cell caspase-3/7 activity stain. View validation data, product information...
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