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PhenoVue 493 Lipid Stain

PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS. View All
PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain

PhenoVue 493 Lipid stain is a lipophilic organic molecule commonly used for localization and quantification of intracellular lipid droplets. ​

PhenoVue 493 Lipid stain exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 493 Lipid stain has a maximum excitation wavelength of 493 nm and a maximum emission wavelength of 504 nm,which makes it an alternative to the similar stain BODIPY™ 493/503.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Feature Specification
Color Green
Filter FITC
Organelle and Cell Compartment Lipid droplets
Request more information

PhenoVue 493 Lipid stain is a lipophilic organic molecule commonly used for localization and quantification of intracellular lipid droplets. ​

PhenoVue 493 Lipid stain exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 493 Lipid stain has a maximum excitation wavelength of 493 nm and a maximum emission wavelength of 504 nm,which makes it an alternative to the similar stain BODIPY™ 493/503.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Product variant
Quantity: 5 x 2 mg
Part #:
CP51
List price
USD 252.01
Your online price:
For research use only. Not for use in diagnostic procedures.
PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain

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Product information

Overview

PhenoVue 493 Lipid stain is a lipophilic fluorescent organic molecule that localizes to polar lipids. It rapidly accumulates in lipid droplets and exhibits enhanced green fluorescence in the presence of neutral lipids like triglycerides and cholesterol esters.

PhenoVue 493 Lipid stain is commonly used for localization and quantification of intracellular lipid droplets which are ​involved in lipid synthesis, metabolism and transportation as well as protein storage and degradation or viral replication, all of which are related to pathophysiology, including dyslipidemia, obesity, lipodystrophy, diabetes, fatty liver diseases, or atherosclerosis.

Typical working concentration: 5 µM (1.31 mg/L)

Equivalent number of microplates:

  • 270 - 830 x 96-well plates
  • 230 - 830 x 384-well plates
  • 430 - 1300 x 1536-well microplates


See Product Information sheet for more information on fixed cell staining.

Specifications

Color
Green
Concentration
1.05 mg/mL (4 mM) in 1.95mL DMSO
Form
Solution in DMSO
Maximum Emission Wavelength (Emmax)
504 nm
Maximum Excitation Wavelength (Exmax)
493 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
FITC
Organelle and Cell Compartment
Lipid droplets
Quantity
5 x 2 mg
Sample Type
Live and fixed samples
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
-16 °C or below, protected from light
Type
Individual reagent

Image gallery

PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 493 Lipid Stain

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Guide to live-cell imaging
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PhenoVue 493 Lipid Stain Product Information Sheet

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PhenoVue Cellular Imaging Reagents Brochure
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