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LANCE Eu-W1024 Anti-Mouse IgG Antibody, 50µg

Europium-labeled anti-mouse IgG, for capturing antibodies in LANCE® TR-FRET assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein Quantification

Europium-labeled anti-mouse IgG, for capturing antibodies in LANCE® TR-FRET assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 50 µg
Part #:
AD0076
List Price
USD 1,486.00
Unit Size: 1 mg
Part #:
AD0077
List Price
USD 12,910.00

Overview

Eu-W1024 labeled anti-mouse IgG antibody for LANCE® assays where analyte concentration < 1-10 nmol/L and assay pH > 7. The universal reagent for detection of mouse antibodies consists of the IgG fraction of anti-mouse antibodies raised in rabbit. There are from 6 to 10 Eu3+ ions per IgG. Interference from human, ox and rat antibodies can usually be removed by adding serum from the other species to the assay buffer.

Generic LANCE® reagents are intended for setting up homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) based assays using Eu-Chelate label as a donor and APC-labeled reagent as an acceptor. Generic reagents facilitate setting up LANCE® assays when there is a limited amount of specific reagents available or the assay reagents are relatively unstable. To enable optimization of both the signal or the noise ratio and signal stability, some of the generic reagents are labeled with two different chelates, W1024 or W8044.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Application
Protein Quantification
Automation Compatible
Yes
Brand
LANCE Ultra
Conjugates
Anti-mouse IgG
Detection Modality
LANCE
Fluorophore
Eu-W1024
Product Group
Antibody
Shipping Conditions
Shipped in Blue Ice
Target Class
Biologics
Target Species
Mouse
Technology
TR-FRET
Unit Size
50 µg

Resources

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Application Note Icon
Application Note
Developing a LANCE TR-FRET assay for screening PD-1 and PD-L1 binding inhibitors.

Here, we demonstrate the utility and benefits of using LANCE Ultra TR-FRET assay technology for identifying and characterizing PD...

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Product Info
LANCE and LANCE Ultra TR-FRET technology product list

LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...

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