Skip to main content
Menu
US

HTRF Tag-Lite Glucagon GLP1 Receptor Cells, 200 Assay Points

Tag-lite cells transiently expressing the Glucagon GLP1 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.

For research use only. Not for use in diagnostic procedures.

Part number: C1TT1GLP1
Unit Size: 200 Assay Points
List price: USD 1,030.00
Your price: Login to view
USD 1,030.00
USD 1,030.00 /each

Overview

The GLP1 receptor is a GPCR which role is to potentiate the glucose-induced secretion of insulin from pancreatic beta cells, increase insulin expression, inhibit beta-cell apoptosis, promote beta-cell neogenesis.

Cells expressing the GLP1 receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.

Specifications

Application
Receptor-Ligand Binding
Brand
Tag-lite
Detection Modality
HTRF
Product Group
Cells
Shipping Conditions
Shipped in Dry Ice
Target Class
GPCR
Technology
TR-FRET
Therapeutic Area
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
Unit Size
200 Assay Points

Video gallery

How it works

Assay principle

Running your GLP1 receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

gpcr-how-it-works-assay-principle-glp1-receptor-c1tt1glp
Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

gpcr-how-it-works-saturation-binding-kd-c1tt1glp
how-it-works-glucagon-glp1-labeled-cells
Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.

gpcr-how-it-works-competitive-binding-ki-c1tt1glp
how-it-works-glucagon-glp1-labeled-cells

 

Assay validation

Kd and Ki validation

Examples of data obtained using Glucagon GLP1 labeled cells and their matching fluorescent ligand (L0030RED). Exendin 4 was used as reference ligand. Results may vary from one HTRF® compatible reader to another.

assay-validation-glucagon-glp1-labeled-cells
assay-validation-glucagon-glp1-labeled-cells

 

Resources

1-2 of 2 Resources
Flyer Icon
Flyer
Download your tag-lite brochure

Tag-lite™ is a cell-based, no-wash alternative to traditional radioisotope assays for studying GPCR-ligand interaction.

Adenosine...

Guide Icon
Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

SDS, COAs, Manuals and more

Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.

IFUs, Manuals & more
Scroll Icon