Tag-lite cells transiently expressing the Angiotensin AT2 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
For research use only. Not for use in diagnostic procedures.
Feature | Specification |
---|---|
Application | Receptor-Ligand Binding |
Tag-lite cells transiently expressing the Angiotensin AT2 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
For research use only. Not for use in diagnostic procedures.
Angiotensin II receptor type 2 or simply AT2 is a GPCR expressed during fetal development. AT2 is involved in vasodilation, inhibition of cell growth, and possibly natriuresis.
Cells expressing the AT2 receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.
Application |
Receptor-Ligand Binding
|
---|---|
Brand |
Tag-lite
|
Detection Modality |
HTRF
|
Product Group |
Cells
|
Shipping Conditions |
Shipped in Dry Ice
|
Target Class |
GPCR
|
Technology |
TR-FRET
|
Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
Unit Size |
200 Assay Points
|
Running your AT2 receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Examples of data obtained using Angiotensin AT2 labeled cells and their matching fluorescent ligand (L0007RED). AT2 was used as reference ligand. Results may vary from one HTRF® compatible reader to another.
Are you looking for resources, click on the resource type to explore further.
G-protein coupled receptors (GPCRs) are crucial transmembrane proteins involved in cellular signal transduction.
This technical...
This guide provides you an overview of HTRF applications in several therapeutic areas.
Tag-lite™ is a cell-based, no-wash alternative to traditional radioisotope assays for studying GPCR-ligand interaction.
Adenosine...
We are here to answer your questions.