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HTRF Tag-Lite Serotonin 5HT4 Receptor Cells, 200 Assay Points

Tag-lite cells transiently expressing the Serotonin 5HT4 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.

For research use only. Not for use in diagnostic procedures.
Part Number: C1TT15HT4
Part Number: C1TT15HT4
Unit Size: 200 Assay Points
  • The 5-HT4 receptor is a postsynaptically located GPCR present at highest densities in the brain. The 5-HT4 GPCR plays a role in learning and memory.

    Cells expressing the 5HT4 receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.

  • Assay Points
    200
    Assay Target Type
    Cells
    Assay Technology
    HTRF
    Brand
    Tag-Lite
    Quantity
    1
    Shelf Life
    540.0 Day(s)
    Therapeutic Area
    Cardiovascular
    Infectious Diseases
    Metabolism/Diabetes
    NASH/Fibrosis
    Neuroscience
    Oncology & Inflammation
    Rare Diseases
    Unit Size
    200 Assay Points
  • Assay principle

    Running your 5HT4 receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

    gpcr-how-it-works-assay-principle
    Saturation binding (KD)

    A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

    gpcr-how-it-works-saturation-binding-kd-c1tt15ht4
    how-it-works-serotonin-5ht4-labeled-cells
    Competitive binding (KI)

    A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.

    gpcr-how-it-works-competitive-binding-ki-c1tt15ht
    how-it-works-serotonin-5ht4-labeled-cells

     

  • Kd and Ki validation

    Examples of data obtained using Serotonin 5HT4 labeled cells and their matching fluorescent ligand (L0043RED). GR-113808 was used as reference ligand. Results may vary from one HTRF® compatible reader to another.

    assay-validation-serotonin-5ht4-labeled-cells
    assay-validation-serotonin-5ht4-labeled-cells

     

Resources

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.