HTRF SARS-Cov2 Spike S1 protein detection kit, 500 Assay Points

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HTRF SARS-Cov2 Spike S1 protein detection kit, 500 Assay Points

This HTRF kit is designed to detect SARS-CoV-2 Spike S1 proteins in cell lysates or cell supernatant, as a marker of viral infection.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature	Specification
Application	Protein Quantification
Dynamic Range	66 - 16,000 pg/mL
Limit of Detection	15 pg/mL
Sample Volume	16 µL

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This HTRF kit is designed to detect SARS-CoV-2 Spike S1 proteins in cell lysates or cell supernatant, as a marker of viral infection.

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Product Variants

Unit Size: 500 Assay Points

Part #:

63ADK114PEG

List Price

USD 2,090.00

Unit Size: 10,000 Assay Points

Part #:

63ADK114PEH

List Price

USD 24,130.00

Product information

Overview

The SARS-CoV-2 Spike S1 protein is one of two part of the virus Spike protein (S). The S1 subunit is responsible for the the virus interaction with host cells' transmembrane Angiotensin-converting enzyme 2 receptors (ACE2), while the S2 subunit is capable of conformational changes that allow the fusion of the viral particle envelope and host cell membrane upon S1/ACE2 binding. As such, the S1 protein is closely investigated for its role in viral infection and is reliable marker of infection as well as a potential vaccine immunogen target.

Specifications

Other Specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Dynamic Range	66 - 16,000 pg/mL
Limit of Detection	15 pg/mL
Product Group	Kit
Sample Volume	16 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Viral Particles
Technology	TR-FRET
Unit Size	500 Assay Points

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How it works

SARS-CoV-2 particles are detected in cell lysates using a sandwich immunoassay involving an anti-S1 monoclonal antibody, labelled with terbium-Cryptate or d2, ensuring assay quality, reproducibility, and signal quality. The specific HTRF signal generated is proportional to the amount of S1 proteins.

The assay protocol, using a 384-well small volume plate or a low volume 96 well plate (20 µL final), is described on the right. 16 µL of cell lysates or control are dispensed directly into the detection plate for detection by HTRF reagents. The antibodies labelled with HTRF donor and acceptor can be pre-mixed and added in a single dispensing step to further streamline the assay procedure (4 µL), or separately (2 µL).

2biomarkers-how-it-works-assay-protocol-spike-s1-protein-kit-63adk114peg-63adk114peh.svg

Assay details

Detection of recombinant SARS-CoV-2 S1 protein

16 µL of recombinant protein in lysis buffer #5 were dispensed into a low volume white microplate before the addition of 4 µL of the premixed HTRF anti SARS-CoV2 capsid detection reagents. The HTRF signal was recorded after ON incubation.

standard curve Spike S1 kit

Sample size	16 µL
Final assay volume	20 µL
Time to result	ON at RT
Detection limit	15 pg/mL
Dynamic range	66 to 16,000 pg/mL
Sample compatibility	Cell-based or cell supernatant