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HTRF PPI Low Ionic Strength Europium Detection buffer, 220 mL

PPI Low Ionic Strength Europium Detection buffer is a spare part intended for use in protein/protein interaction assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Part number: 61DB11RDF
Unit Size: 220 mL
List price: USD 545.00
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USD 545.00
USD 545.00 /each

Overview

This Low Ionic Strength Eu detection buffer is specifically designed for
protein-protein interaction studies. It has been optimized for maximum
performance when the studied protein-protein interaction involves a Europium
cryptate conjugate in the assay and is sensitive to high ionic strength.

Specifications

Application
Protein-Protein Interaction
Brand
HTRF
Detection Modality
HTRF
Product Group
Buffer
Shipping Conditions
Shipped Ambient
Target Class
Binding Assay
Technology
TR-FRET
Unit Size
220 mL

Assay validation

Evaluation of the two PPI Eu buffers in protein/protein interaction assays

The two Europium PPI detection buffers were evaluated in two different Protein/Protein interaction assays.

The IC50 values corresponding to the inhibition of the two interactions (A/B & C/D) remained comparable in both PPI Eu detection buffers.

The Protein A/ Protein B interaction displayed the same Signal to Background (S/B) in the PPI Eu detection buffer and the PPI Low Ionic Strength Eu detection buffer.

However, the S/B of the Protein C/ Protein D interaction was significantly improved in the new PPI Low Ionic Strength Eu detection buffer, suggesting that the PPI Low Ionic Strength Eu detection buffer can maximize assay performance when the studied protein-protein interaction is sensitive to high ionic strength.

Evaluation of the two PPi Eu buffers in protein/protein interaction assays
Evaluation of the two PPi Eu buffers in protein/protein interaction assays

 

Resources

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SDS, COAs, Manuals and more

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