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HTRF PolyGT-Biotin substrate, 5,000 Assay Points

PolyGT-Biotin has been shown to be a substrate for tyrosine-specific protein kinases.

For research use only. Not for use in diagnostic procedures.
Part Number: 61GT0BLA
Part Number: 61GT0BLA
Unit Size: 5,000 Assay Points
  • Poly-(Glu,Tyr) (4:1) is a random copolymer which has been shown to be a substrate for tyrosine-specific protein kinases. Poly-(GT)-biotin may be used with Streptavidin-XL665 and Anti-phosphotyrosine-cryptate for easily optimized HTRF® tyrosine kinase assays. HTRF enables to perform assay at physiological ATP concentrations.

  • Assay Points
    5000
    Assay Target Type
    Other reagent
    Assay Technology
    HTRF
    Brand
    HTRF
    Quantity
    1
    Therapeutic Area
    Cardiovascular
    Infectious Diseases
    Inflammation
    Metabolism/Diabetes
    NASH/Fibrosis
    Neuroscience
    Oncology & Inflammation
    Rare Diseases
    Unit Size
    5,000 Assay Points
  • Assay principles

    HTRF Tyrosine kinase assay use typically a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and PolyGT-Biotin as substrate. The signal is proportional to the concentration of phospho-tyrosine.

    HTRF PolyGT-Biotin substrate 1.svg

     

    Assay protocol

    HTRF tyrosine kinase assay have two basic phases: 1. Enzymatic step: biotinylated PolyGT is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated PolyGT is then detected by the addition of Streptavidin-XL665 and a Europium-cryptate labeled anti-phosphotyrosine antibody (MAb PY20-Eu cryptate or MAb PT66-Eu cryptate) prepared in a buffer containing EDTA to stop the enzymatic reaction.

    HTRF PolyGT-Biotin substrate 2.svg

     

Resources

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.