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HTRF Anti-p-Thr pAb Eu-Conjugate, 500 Assay Points

PAb Antiphosthreonine-Eu cryptate enables the rapid detection of phosphorylation of peptides or proteins on Thr in a biochemical kinase assay format.

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Feature	Specification
Application	Biochemical Enzymatic Assay

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PAb Antiphosthreonine-Eu cryptate enables the rapid detection of phosphorylation of peptides or proteins on Thr in a biochemical kinase assay format.

View product information

Product Variants

Part #:

61PTRKAE

List Price

USD 753.58

Your online price:

Part #:

61PTRKAZ

List Price

USD 1,839.86

Your online price:

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

Product information

Overview

The Anti-phospho threonine-Eu cryptate is a labeled polyclonal antibody. It is highly specific for the presence of phosphothreonine in peptides or proteins and shows no cross-reactivity with the corresponding non-phosphorylated threonine residues. This Anti-Phosphothreonine antibody does not react with phosphotyrosine or phosphoserine. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.

Specifications

Other Specifications

Application	Biochemical Enzymatic Assay
Brand	HTRF
Detection Modality	HTRF
Product Group	Fluorescent Reagent
Shipping Conditions	Shipped in Dry Ice
Target Class	Kinases
Technology	TR-FRET
Therapeutic Area	Cardiovascular Infectious Diseases Inflammation Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size	500 assay points

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How it works

Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

HTRF PAb Antiphosphothreonine-Eu cryptate 1.svg

Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and PAb Antiphosphothreonine-Eu cryptate prepared in a buffer containing EDTA to stop the enzymatic reaction.