Skip to main content
Menu
US

HTRF Mouse IL-1β Detection Kit, 500 Assay Points

HTRF mouse IL-β kit is designed quantification of mouse IL-β release in cell supernatant.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW when you buy online.
Promo code: REV15NOW
Offer valid until 11/6. Terms and conditions apply.
Product Variants
Part number: 62MIL1BPEG
Unit Size: 500 Assay Points
List price: USD 1,103.00
Your price: Login to view
USD 1,103.00
USD 1,103.00 /each
Part number: 62MIL1BPEH
Unit Size: 10,000 Assay Points
List price: USD 10,290.00
Your price: Login to view
USD 0.00
USD 10,290.00 /each

Overview

IL1 beta, also known as leukocytic pyrogen or mononuclear cell factor, is a pro-inflammatory cytokine of IL1 family. IL1 beta is considered to be a major endogenous pyrogen and induces prostaglandin synsis, T cell activation, B cell activation, and subsequent antibody production. It is also a known promoter of T cell differentiation into Th17.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma. When assaying, always follow recommended protocol and avoid highly haemolyzed samples.

Specifications

Application
Protein Quantification
Assay Target Class
Cytokines
Assay Technology
TR-FRET
Brand
HTRF
Detection Method
HTRF
Product Group
Kit
Sample Volume
16 µL
Shipping Conditions
Shipped in Dry Ice
Therapeutic Area
NASH/Fibrosis
Oncology & Inflammation
Unit Size
500 Assay Points

Video gallery

Citations

How it works

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

mouse-il1-beta-kit

 

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

Analytical performance

Intra and inter assay

Intra-assay (n=24)

Sample Mean [IL1ß] (pg/mL) CV
1 100 6%
2 800 2%
3 3200 2%
  Mean CV 3%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample [IL1ß] (pg/mL) Mean (delta R) CV
1 170 272 2%
2 612 1023 4%
3 2211 3009 1%
    Mean CV 2%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample

Resources

1-2 of 2 Resources
Brochure Icon
Brochure
HTRF assays and reagents catalog

Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...

Guide Icon
Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

SDS, COAs, Manuals and more

Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.

Safety data sheet
Certificate of analysis
+ Show more
IFUs, Manuals & more
Scroll Icon