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HTRF mAb Anti-Phospho-P38 MAPK (Thr180/Tyr182) Eu-Conjugate, 500 Assay Points

MAb Anti-phospho P38-Eu cryptate enables the rapid detection of p38 MAPK phosphorylation on Thr180 & Tyr182 in a biochemical kinase assay format.

For research use only. Not for use in diagnostic procedures.

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Feature Specification
Application Biochemical Enzymatic Assay

MAb Anti-phospho P38-Eu cryptate enables the rapid detection of p38 MAPK phosphorylation on Thr180 & Tyr182 in a biochemical kinase assay format.

For research use only. Not for use in diagnostic procedures.

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
61P04KAE
List Price
USD 622.06
Unit Size: 10,000 Assay Points
Part #:
61P04KAZ
List Price
USD 2,285.00

Overview

The Anti-phospho p38-Eu cryptate is a labeled MAb enabling the detection of p38 MAPK only when phosphorylated at Thr180 & Tyr 182. It does not cross-react with the corresponding phosphorylated forms of either p44/42 MAPK or SAPK/JNK. It does not detect non-phosphorylated p38 MAPK. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.

Specifications

Application
Biochemical Enzymatic Assay
Brand
HTRF
Detection Modality
HTRF
Molecular Modification
Phosphorylation
Product Group
Fluorescent Reagent
Shipping Conditions
Shipped in Dry Ice
Target Class
Kinase
Technology
TR-FRET
Therapeutic Area
Cardiovascular
Infectious Diseases
Inflammation
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
Unit Size
500 Assay Points

Video gallery

How it works

Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

HTRF MAb Anti-phospho P38-Eu cryptate 1a.svg

 

Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb Anti-phospho P38-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.

HTRF MAb Anti-phospho P38-Eu cryptate 1b.svg

 

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