HTRF MAb Anti-phospho P38-Eu cryptate, 500 Assay Points

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HTRF MAb Anti-phospho P38-Eu cryptate, 500 Assay Points

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MAb Anti-phospho P38-Eu cryptate enables the rapid detection of p38 MAPK phosphorylation on Thr180 & Tyr182 in a biochemical kinase assay format.

Product information

For research use only. Not for use in diagnostic procedures.

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Product Variants

partnumber

Part number: 61P04KAE

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Unit Size: 500 Assay Points

List price: USD 622.06

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USD 622.06

USD 622.06 /each

partnumber

Part number: 61P04KAZ

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Unit Size: 10,000 Assay Points

List price: USD 2,285.00

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USD 2,285.00 /each

Product information

Overview

The Anti-phospho p38-Eu cryptate is a labeled MAb enabling the detection of p38 MAPK only when phosphorylated at Thr180 & Tyr 182. It does not cross-react with the corresponding phosphorylated forms of either p44/42 MAPK or SAPK/JNK. It does not detect non-phosphorylated p38 MAPK. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.

Specifications

Other Specifications

Assay Points	500
Assay Target Type	Fluorescent reagent
Assay Technology	HTRF
Brand	HTRF
Quantity	1
Shipping Conditions	Shipped in Dry Ice
Therapeutic Area	Cardiovascular Infectious Diseases Inflammation Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size	500 Assay Points

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How it works

Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

HTRF MAb Anti-phospho P38-Eu cryptate 1a.svg

Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb Anti-phospho P38-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.