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HTRF mAb Anti-GST XL665-Conjugate, 1,000 Assay Points

XL665-labeled anti-GST antibody for capturing GST-tagged proteins in protein/protein interaction assays.

For research use only. Not for use in diagnostic procedures.

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Feature Specification
Application Protein-Protein Interaction

XL665-labeled anti-GST antibody for capturing GST-tagged proteins in protein/protein interaction assays.

For research use only. Not for use in diagnostic procedures.

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 1,000 Assay Points
Part #:
61GSTXLF
List Price
USD 262.25
Unit Size: 5,000 Assay Points
Part #:
61GSTXLA
List Price
USD 609.47
Unit Size: 20,000 Assay Points
Part #:
61GSTXLB
List Price
USD 1,911.00

Overview

MAb Anti GST-XL665 is an IgG2a raised against Glutathione S-transferase labeled with XL665. It has been shown to react with GST-tagged fusion proteins from a large number of expressing vectors.

This reagent can be used in both biochemical and cellular formats to study a wide variety of interactions: protein/protein, protein/peptide, protein/DNA, protein/RNA, protein/carbohydrate, protein/small molecule, receptor/ligand.

HTRF can detect a broad range of affinity constants ranging from picomolar to low millimolar.

Specifications

Application
Protein-Protein Interaction
Brand
HTRF
Detection Modality
HTRF
Product Group
Fluorescent Reagent
Shipping Conditions
Shipped Ambient
Target Class
Binding Assay
Technology
TR-FRET
Therapeutic Area
Cardiovascular
Infectious Diseases
Inflammation
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
Unit Size
1,000 Assay Points

Video gallery

Citations

How it works

Assay principle

In an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the signal is proportional to the binding of the 2 partners. In the example shown here: MAb Anti GST-XL665 binds to the GST tagged partner A while partner B* binds to a specific Ab labeled with an HTRF donor. *partner B can also be biotinylated, tagged, Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with donor for the detection.

 

HTRF MAb Anti GST-XL665 1.svg
Assay protocol

The example on the right describes the protocol using a 20 µL final assay volume for detecting an interaction between a GST-tagged partner A and a non-tagged partner B*. Dispense the 2 partners (10 µL), incubate, add MAb Anti GST-XL665 (5 µL) and anti-partner B labeled with donor (5 µL), incubate and read. *partner B can also be biotinylated, tagged, Fc fused or directly labeled. In these cases, use the corresponding HTRF reagent (anti-Tag, anti species, protA, Streptavidin) labeled with donor for the detection.

HTRF MAb Anti GST-XL665 2.svg

 

Assay details

Build HTRF interaction assays for your specific application

Reagents are sold by the number of tests (20 µL reactions). Antibodies conjugated to XL665 or d2 are supplied on the basis of 20 ng of antibody per well. The amount of active moiety per vial is also provided (as well as the number of tracers per vial - see product description sheet). The active moiety is defined as the active part of a conjugate (e.g. antibody).

How do the number of tests relate to active moiety?

The average conjugate quantity per well reflects overall biological material content. Using the active moiety amount is generally preferred to the quantity of total conjugate. For Cryptate and d2 conjugates, the total conjugate amount equals that of the active moiety, since the molecular weight of the label is negligible. This is not the case for XL665 labeled entities for which the quantity of total conjugate will vary depending on the final molar ratio of the XL665 conjugate, however, the amount of active moiety, provided by Revvity, is constant and based on the number of tests ordered.

HTRF MAb Anti GST-XL665 3.svg

 

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