Skip to main content
Menu
US

HTRF LTC4 Detection Kit, 500 Assay Points

The Leukotriene C4 kit is designed for the accurate quantification of LTC4.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: 64LC4PEG
Unit Size: 500 Assay Points
List price: USD 1,039.00
Your price: Login to view
USD 1,039.00
USD 1,039.00 /each
Part number: 64LC4PEH
Unit Size: 10,000 Assay Points
List price: USD 11,540.00
Your price: Login to view
USD 0.00
USD 11,540.00 /each

Overview

LTC4 is a major leukotriene product that is enzymatically derived from the arachidonic acid cascade. Leukotriene synthesis operates via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA4 with 5(S)-HpETE as an intermediate. The conjugation of glutathione to LTA4 results in the formation of LTC4, which is then rapidly metabolized to LTD4 and LTE4. LTC4 has been described as a potent mediator of inflammatory diseases and immunoregulation. The LTC4 kit enables the monitoring of LTC4 produced by cells and can be used to study the LTC4-hydrolase.

Specifications

Application
Protein Quantification
Brand
HTRF
Detection Modality
HTRF
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Dry Ice
Target Class
Biomarkers
Technology
TR-FRET
Therapeutic Area
Cardiovascular
Neuroscience
Oncology & Inflammation
Unit Size
500 Assay Points

Video gallery

How it works

Assay principle

The LTC4 kit is a competitive immunoassay for the measurement of LTC4 in a range of biological formats. The assay uses an Eu3+-Cryptate conjugated antibody specific to LTC4 and XL665 coupled LTC4. LTC4 contained in the sample competes with XL665-labelled LTC4 for binding to anti-LTC4 Eu Cryptate.

Biomarkers how it works assay principle ltb4 64lc4peg 64lc4peh
Assay protocol

The LTC4 assay features a streamlined protocol with only 1 incubation step after sample and LTC4 detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.

Biomarkers how it works assay protocol ltb4 64lc4peg 64lc4peh

 

Assay details

Key features
  LTC4
Incubation 1h at RT
Assay range 0.1 5000 ng/mL
EC50 2.7 ng/mL
Detection limit 0.1 ng/mL
Specificity No cross reactivity with LTA4, LTB4

 

64LC4PEG specifications ltc4-1

 

Resources

1-3 of 3 Resources
Brochure Icon
Brochure
HTRF assays and reagents catalog

Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...

Guide Icon
Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

Flyer Icon
Flyer
Reagent solutions for autoimmunity research.

Advance your autoimmune disease research and benefit from Revvity broad offering of reagent technologies

SDS, COAs, Manuals and more

Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.

Safety data sheet
+ Show more
Certificate of analysis
+ Show more
IFUs, Manuals & more
Scroll Icon