The Leukotriene C4 kit is designed for the accurate quantification of LTC4.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
LTC4 is a major leukotriene product that is enzymatically derived from the arachidonic acid cascade. Leukotriene synthesis operates via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA4 with 5(S)-HpETE as an intermediate. The conjugation of glutathione to LTA4 results in the formation of LTC4, which is then rapidly metabolized to LTD4 and LTE4. LTC4 has been described as a potent mediator of inflammatory diseases and immunoregulation. The LTC4 kit enables the monitoring of LTC4 produced by cells and can be used to study the LTC4-hydrolase.
Application |
Protein Quantification
|
---|---|
Brand |
HTRF
|
Detection Modality |
HTRF
|
Product Group |
Kit
|
Sample Volume |
10 µL
|
Shipping Conditions |
Shipped in Dry Ice
|
Target Class |
Biomarkers
|
Technology |
TR-FRET
|
Therapeutic Area |
Cardiovascular
Neuroscience
Oncology & Inflammation
|
Unit Size |
500 Assay Points
|
The LTC4 kit is a competitive immunoassay for the measurement of LTC4 in a range of biological formats. The assay uses an Eu3+-Cryptate conjugated antibody specific to LTC4 and XL665 coupled LTC4. LTC4 contained in the sample competes with XL665-labelled LTC4 for binding to anti-LTC4 Eu Cryptate.
The LTC4 assay features a streamlined protocol with only 1 incubation step after sample and LTC4 detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.
LTC4 | |
---|---|
Incubation | 1h at RT |
Assay range | 0.1 5000 ng/mL |
EC50 | 2.7 ng/mL |
Detection limit | 0.1 ng/mL |
Specificity | No cross reactivity with LTA4, LTB4 |
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
This guide provides you an overview of HTRF applications in several therapeutic areas.
Advance your autoimmune disease research and benefit from Revvity broad offering of reagent technologies
Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.
We are here to answer your questions.