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HTRF mAb Human IgG-λ Detection Kit, 500 Assay Points

The Human IgG Lambda MAb kit is designed for the fast quantification of human IgGs Lambda light chains.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein Quantification
Sample Volume 10 µL

The Human IgG Lambda MAb kit is designed for the fast quantification of human IgGs Lambda light chains.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
64LAMPEG
List Price
USD 778.36
Unit Size: 10,000 Assay Points
Part #:
64LAMPEH
List Price
USD 6,819.00

Overview

Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The human IgG Lambda MAb kit enables a rapid quantification of human IgG Lambda light chains in cell supernatant. The assay is compatible with natural IgG as well as recombinant antibodies.

Specifications

Application
Protein Quantification
Brand
HTRF
Detection Modality
HTRF
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Dry Ice
Target Class
Biologics
Target Species
Human
Technology
TR-FRET
Therapeutic Area
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Rare Diseases
Unit Size
500 Assay Points

Video gallery

How it works

Assay principle

The human IgG Lambda MAb kit is detected in a sandwich assay format using 2 different specific antibodies. The anti-(h)-IgG Lambda antibody is labelled with d2 (acceptor) and the anti(h)-Fc antibody is labelled with Eu3+-Cryptate (donor). The two antibodies bind to the (h)IgG Kappa present in the sample, thereby generating FRET. The specific signal modulates positively in proportion to (h)IgG Lambda.

biomarkers-assay-principle-human-igg-lambda-mab-64lampeg

 

Assay protocol

The human IgG Lambda MAb assay features a streamlined protocol with only 1 incubation step after sample and IgG lambda MAb detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.

biomarkers-assay-protocol-human-igg-lambda-mab-64lampeg

 

Assay details

Key features
  Lambda
Detection limit 0.4 ng/mL
Dynamic range 0.8 to 200 ng/mL
Cross reactivity

0% Human Kappa

0% Mouse Lambda

0% Human IgM

 

specifications-lambda-mab-1

 

Resources

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

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