HTRF KRAS G12C / SOS1 Binding Kit Standard

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HTRF KRAS G12C / SOS1 Binding Kit Standard

The KRAS/SOS1 binding Standard is intended for use with the KRAS WT/SOS1 and KRAS G12C/SOS1 binding kits.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature	Specification
Application	Protein-Protein Interaction

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The KRAS/SOS1 binding Standard is intended for use with the KRAS WT/SOS1 and KRAS G12C/SOS1 binding kits.

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Product Variant

Unit Size: 1 Vial

Part #:

64KRASPPICDA

List Price

USD 366.10

Product information

Overview

KRAS/SOS1 binding standard can be used as a standard to control assay reproducibility. KRAS/SOS1 binding d is intended for use with the KRAS WT/SOS1 and the KRAS G12C/SOS1 kits.

Specifications

Other Specifications

Application	Protein-Protein Interaction
Brand	HTRF
Detection Modality	HTRF
Product Group	Standard
Shipping Conditions	Shipped in Dry Ice
Target Class	Binding Assay
Technology	TR-FRET
Therapeutic Area	Oncology & Inflammation
Unit Size	1 Vial

Video gallery

How it works

Assay principle

The KRAS/SOS1 PPI assays include tagged human recombinant partners (KRAS WT or G12C and SOS1) and labelled anti-tag reagents for HTRF detection. Without any inhibitor, KRAS loaded with GTP binds to SOS1 , and the binding of each detection reagent to its tagged target generates an HTRF signal. In the presence of KRAS/SOS1 inhibitors or GTP competitors, the HTRF signal decreases.

Assay protocol

The Human KRAS/SOS1 binding assay can be run in a 96- or 384-well low volume white plate (20 µL final). As described here, compounds or standards are dispensed directly into the assay plate. The pre-mixed GTP and human Tag1-KRAS protein is then added, together with the human Tag2-SOS1 protein, followed by the dispensing of the HTRF reagents, the anti-Tag2 antibody labeled with Terbium cryptate and the anti-Tag1 antibody labeled with XL665. The reagents labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally.