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HTRF Human Perforin-1 Detection Kit, 500 Assay Points

The Human Perforin-1 kit is designed for the rapid detection of human Perforin-1 in cell supernatant and whole cells.

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Feature	Specification
Application	Protein Quantification
Sample Volume	16 µL

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The Human Perforin-1 kit is designed for the rapid detection of human Perforin-1 in cell supernatant and whole cells.

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Product Variants

Part #:

63ADK100PEG

List Price

USD 1,260.82

Your online price:

Part #:

63ADK100PEH

List Price

USD 14,983.96

Your online price:

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

Product information

Overview

Perforin (also called Cytolysin or Lymphocyte pore-forming protein) is found in cytotoxic lymphocytes, NK cells (natural killer), and cytotoxic T cells. Perforin creates a pore in the cell membrane to allow Granzyme B to enter the target cells cytoplasm and triggers apoptosis through caspase activation. This kit is designed for the rapid detection of human Perforin in cell supernatant and whole cells. The assay enables high throughput without any washing steps, thereby saving time compared to ELISA.

Specifications

Other Specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	16 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Biomarkers
Target Species	Human
Technology	TR-FRET
Therapeutic Area	Oncology & Inflammation
Unit Size	500 assay points

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How it works

Assay principle

Human Perforin is measured with a sandwich immunoassay involving two specific anti-human Perforin antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of Perforin present in the sample.

Assay protocol

The assay protocol, using a 384-well small volume white plate or a Revvity low volume 96 well plate (20 µL final), is described on the right. 16 µL of cell supernatant, sample, or standard are dispensed directly into the detection plate for detection by HTRF reagents. The antibodies labelled with HTRF donor and acceptor can be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

Analytical performance

Sample size	16 µL
LOD &LOQ (in diluent)	0.1 ng/mL &0.25 ng/mL
Assay range	0.25 – 100 ng/mL
Time to result	ON at RT

Assay validation

Detection of perforin on cell supernatant (two-plate-protocol)

Different cell densities of TALL-104 cells (Effector cells) were plated in suspension under 50 µL in 96-well plate. TALL-104 cells were cultured for 24 hours at 37 °C - 5% CO2. After culture, 16 µL of cell supernatants cells were transferred into a 384-well sv white microplate and 4 µL of the HTRF Perforin and Granzyme B detection reagentswere added. The HTRF signal was recorded after an overnight incubation at room temperature.