The HTRF human IL31 kit is designed for the quantification of human IL31 release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
IL31 is preferentially produced by TH2 cells, and acts on activated monocytes and unstimulated epilial cells. It could be involved in skin inflammation, in particular pruritus (itching).
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma. When assaying, always follow recommended protocol and avoid highly haemolyzed samples.
Assay Points |
500
|
---|---|
Assay Technology |
HTRF
|
Brand |
HTRF
|
Product Group |
Kit
|
Quantity |
1
|
Shipping Conditions |
Shipped in Dry Ice
|
Therapeutic Area |
Oncology & Inflammation
|
Unit Size |
500 Assay Points
|
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting,
Revvity also worked with Myassays.com to help you in your data analysis. you’ll be able to access a free online software to run your IL31 analysis.
Sample size | 16 µL |
---|---|
Final assay volume | 20 µL |
Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
LOD &OQ (in Diluent) | 18 pg/mL &87 pg/mL |
Range | 87 20,000 pg/mL |
Time to result | Overnight at RT |
Species | Human only |
Intra-assay (n=24)
Sample | Mean [IL31] (pg/mL) | CV |
---|---|---|
1 | 199 | 7% |
2 | 9888 | 6% |
3 | 20883 | 5% |
Mean CV | 6% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
Sample | [IL31] (pg/mL) | Mean (delta R) | CV |
---|---|---|---|
1 | 388 | 487 | 19% |
2 | 1878 | 1674 | 11% |
3 | 9091 | 4007 | 4% |
Mean CV | 11% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
This guide provides you an overview of HTRF applications in several therapeutic areas.
Cytokines play a vital role in both innate and adaptive immunity and are known for their ability to exert diverse functions on...
Advance your autoimmune disease research and benefit from Revvity broad offering of reagent technologies
Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.
We are here to answer your questions.