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HTRF Human IL-23 Detection Kit, 500 Assay Points
HTRF Human IL-23 Detection Kit, 500 Assay Points
PHOTO HUMAN IL23 KIT
The HTRF human IL23 kit is designed for the quantification of human IL23 release in cell supernatant.
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| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 16 µL |
The HTRF human IL23 kit is designed for the quantification of human IL23 release in cell supernatant.
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Product Variants
Unit Size: 500 assay points
Part #:
62HIL23PEG
List Price
USD 1,149.33
Your online price:
Unit Size: 10,000 assay points
Part #:
62HIL23PEH
List Price
USD 10,722.18
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Human IL-23 Detection Kit, 500 Assay Points
PHOTO HUMAN IL23 KIT
HTRF Human IL-23 Detection Kit, 500 Assay Points
Product information
Overview
IL23 is part of the response against infection as it upregulates the expression of MMP9 (matrix metalloprotease 9), increases angiogenesis, and reduces CD8 T cell infiltration into tumors. In oncology and immuno-oncology, IL23 has been studied for its effects on T cell infiltration and its promotion on TH17 cell development. IL23 is a heterodimeric cytokine composed of p19 and p40 subunits, the latter being shared with IL12. Cisbio's Human IL23 assay is highly specific for IL23 and does not cross react with IL12.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
16 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Cytokines
|
| Target Species |
Human
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Oncology & Inflammation
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Human IL-23 Detection Kit, 500 Assay Points
HTRF Human IL-23 Detection Kit, 500 Assay Points
How it works
Assay Principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting,
Revvity also worked with Myassays.com to help you in your data analysis, you’ll be able to access a free online software to run your IL23 analysis.
Assay details
Technical specifications of human IL23 kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD &LOQ (in Diluent) | 30 pg/mL &92 pg/mL |
| Range | 92 30,000 pg/mL |
| Time to result | 3h at 4°C |
| Species | Human only |
Analytical performance
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [IL23] (pg/mL) | CV |
|---|---|---|
| 1 | 200 | 10% |
| 2 | 10,000 | 2% |
| 3 | 20,000 | 2% |
| Mean CV | 5% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
| Sample | [IL23] (pg/mL) |
Mean (delta R) | CV |
|---|---|---|---|
| 1 | 307 |
259 |
12% |
| 2 | 1920 |
1534 |
7% |
| 3 | 12,000 |
8601 |
3% |
| Mean CV | 7% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample
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