HTRF Human IL-18 Detection Kit, 500 Assay Points

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HTRF Human IL-18 Detection Kit, 500 Assay Points

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The HTRF human IL18 kit is designed for the quantification of human IL18 release in cell supernatant.

Product information

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Product Variants

partnumber

Part number: 62HIL18PEG

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Unit Size: 500 Assay Points

List price: USD 1,103.00

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USD 1,103.00

USD 1,103.00 /each

partnumber

Part number: 62HIL18PEH

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Unit Size: 10,000 Assay Points

List price: USD 10,290.00

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USD 10,290.00 /each

Product information

Overview

Also called IGIF (interferon gamma inducing factor), IL18 is a pro-inflammatory cytokine secreted mainly by macrophages. Its biological activity is close to that of IL12, and it can enhance NK cell activity as well as induce IFNg secretion by PBMC. An important role of IL18 is eliminating VEGF from eye, thus protecting retina. It is used toger with anti-VEGF antibodies in treatment of AMD (age-related macular degeneration). In addition to this physiological role, IL18 dysregulation leads to severe inflammatory reactions.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.

Specifications

Other Specifications

Assay Points	500
Assay Technology	HTRF
Brand	HTRF
Product Group	Kit
Quantity	1
Shipping Conditions	Shipped in Dry Ice
Unit Size	500 Assay Points

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How it works

Assay Principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases. To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page. Revvity also worked with Myassays.com to help you in your data analysis. you’ll be able to access a free online software to run your IL18 analysis.

Assay details

Technical specifications of human IL18 kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)	18 pg/mL &43 pg/mL
Range	43 8,000 pg/mL
Time to result	1h at RT
Calibration	NIBSC (03/200) value (IU/mL) = 5 x HTRF hIL18 value (pg/mL)
Species	Human only