HTRF Human IL-17 Detection Kit, 96 Assay Points

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HTRF Human IL-17 Detection Kit, 96 Assay Points

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The HTRF human IL17 kit is designed for the quantification of human IL17 release in cell supernatant.

Product information

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Product Variants

partnumber

Part number: 62HIL17PET

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Unit Size: 96 Assay Points

List price: USD 652.48

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USD 652.48

USD 652.48 /each

partnumber

Part number: 62HIL17PEG

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Unit Size: 500 Assay Points

List price: USD 1,103.00

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USD 0.00

USD 1,103.00 /each

partnumber

Part number: 62HIL17PEH

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Unit Size: 10,000 Assay Points

List price: USD 10,290.00

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USD 10,290.00 /each

Product information

Overview

IL17 is a pro-inflammatory cytokine induced by IL23 and produced by helper T cells. IL17 is part of response to fungi and bacteria, and promotes release of or pro-inflammatory cytokines and chemokines. Due to its role as a mediator of inflammatory responses, IL17 has been linked to allergic responses and autoimmune diseases like asthma, psoriasis, or IBD (inflammatory bowel disease). In immuno-oncology, IL17 is main cytokine released by differentiated TH17 cells.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.

Specifications

Other Specifications

Assay Points	96
Assay Technology	HTRF
Brand	HTRF
Product Group	Kit
Quantity	1
Shipping Conditions	Shipped in Dry Ice
Therapeutic Area	NASH/Fibrosis Oncology & Inflammation
Unit Size	96 Assay Points

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How it works

Assay Principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases. To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page. Revvity also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL17 analysis.

Assay details

Technical specifications of human IL17 kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)	7 pg/mL &33 pg/mL
Range	33 10,000 pg/mL
Time to result	Overnight at RT
Calibration	NIBSC (01/420) value (IU/mL) = 0,02 x HTRF hIL17 value (pg/mL)
Species	Human only