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HTRF Human IL-1β Detection Kit, 96 Assay Points
HTRF Human IL-1β Detection Kit, 96 Assay Points
PHOTO HUMAN IL1 BETA KIT
HTRF human IL1-β kit is designed quantification of human IL1-β release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 16 µL |
HTRF human IL1-β kit is designed quantification of human IL1-β release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Unit Size: 96 Assay Points
Part #:
62HIL1BPET
List Price
USD 652.48
Unit Size: 500 Assay Points
Part #:
62HIL1BPEG
List Price
USD 1,103.00
Unit Size: 10,000 Assay Points
Part #:
62HIL1BPEH
List Price
USD 10,290.00
HTRF Human IL-1β Detection Kit, 96 Assay Points
PHOTO HUMAN IL1 BETA KIT
HTRF Human IL-1β Detection Kit, 96 Assay Points
Product information
Overview
IL1 beta, also known as leukocytic pyrogen or mononuclear cell factor, is a pro-inflammatory cytokine of IL1 family. IL1 beta is considered to be a major endogenous pyrogen and induces prostaglandin synsis, T cell activation, B cell activation, and subsequent antibody production. It is also a known promoter of T cell differentiation into Th17.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
16 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Cytokines
|
| Target Species |
Human
|
| Technology |
TR-FRET
|
| Therapeutic Area |
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
|
| Unit Size |
96 Assay Points
|
Video gallery
HTRF Human IL-1β Detection Kit, 96 Assay Points
HTRF Human IL-1β Detection Kit, 96 Assay Points
Citations
How it works
Assay Principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases. To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page. Revvity also worked with Myassays.com to help you in your data analysis. you’ll be able to access a free online software to run your IL1 beta analysis.
Assay details
Technical specifications of human IL1 beta kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD &LOQ (in Diluent) | 5 pg/mL &39.4 pg/mL |
| Range | 39.4 6,500 pg/mL |
| Time to result | Overnight at RT |
| Calibration | NIBSC (86/680) value (IU/mL) = 0,1 x HTRF hIL1ß value (pg/mL) |
| Species | Human only |
Analytical performance
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [IL1ß] (pg/mL) | CV |
|---|---|---|
| 1 | 65 | 18% |
| 2 | 705 | 9% |
| 3 | 5030 | 4% |
| Mean CV | 10% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
| Sample | [IL1ß] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 159 | 378 | 15% |
| 2 | 702 | 1742 | 13% |
| 3 | 3095 | 7736 | 12% |
| Mean CV | 13% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
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