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HTRF Human IFNα Detection Kit, 500 Assay Points

The HTRF human IFN alpha kit is designed for the quantification of human IFN alpha release in cell supernatant.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: 62HIFNAPEG
Unit Size: 500 Assay Points
List price: USD 1,103.00
Your price:
USD 1,103.00
USD 1,103.00 /each
Part number: 62HIFNAPEH
Unit Size: 10,000 Assay Points
List price: USD 10,290.00
Your price:
USD 0.00
USD 10,290.00 /each

Overview

Mainly produced by leukocytes, IFN alpha is involved in innate immunity response to viral infection. IFN alpha activates several intracellular pathways in target cells, which explains its pleiotropic effects on cell differentiation, growth, and immunoregulation.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.

Specifications

Assay Points
500
Assay Target Type
Kit
Assay Technology
HTRF
Brand
HTRF
Quantity
1
Shipping Conditions
Shipped in Dry Ice
Therapeutic Area
Oncology & Inflammation
Unit Size
500 Assay Points

Video gallery

How it works

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

cytokines-how-it-works-assay-principle-human-ifn-alpha-62hifnapeg-62hifnapeh.svg
Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases. 
 

Assay details

Technical specifications of human IFN alpha kit
Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent) 11 pg/mL &36 pg/mL
Calibration NIBSC (94/784) value (IU/mL) = 0,9 x HTRF hIFNa HTRF
Range 36  4,000 pg/mL
Time to result 2h at RT
Species Human only

 

Analytical performance

Intra and inter assay

Intra-assay (n=24)

Sample Mean [IFNa] (pg/mL) CV
1 150 11%
2 1500 2%
3 3000 5%
  Mean CV 6%


Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample [IFNa] (pg/mL) Mean (delta R) CV
1 47 268 9%
2 206 1150 5%
3 1905 4227 4%
    Mean CV 6%


Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

Resources

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