Skip to main content
Menu
US

HTRF Human G-CSF Detection Kit, 500 Assay Points

The HTRF human G-CSF kit is designed for the quantification of human G-CSF release in cell supernatant.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: 62HGCSFPEG
Unit Size: 500 Assay Points
List price: USD 1,103.00
Your price:
USD 1,103.00
USD 1,103.00 /each
Part number: 62HGCSFPEH
Unit Size: 10,000 Assay Points
List price: USD 10,290.00
Your price:
USD 0.00
USD 10,290.00 /each

Overview

Also known as CSF3 (colony-stimulating factor 3), G-CSF is a hematopoietic cytokine. It acts on precursor cells in bone marrow to initiate ir proliferation and differentiation into mature granulocytes, on hematopoietic stem cells to induce ir mobilization in blood stream, and on neurons to promote neurogenesis and block apoptosis. In myocardial infarction or ischemia, G-CSF promotes T cells' immune tolerance and tissue recovery. absence of a functional G-CSF receptor is associated with congenital neutropenia.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.

Specifications

Assay Points
500
Assay Target Type
Kit
Assay Technology
HTRF
Brand
HTRF
Quantity
1
Shipping Conditions
Shipped in Dry Ice
Therapeutic Area
Cardiovascular
Neuroscience
Oncology & Inflammation
Unit Size
500 Assay Points

Video gallery

How it works

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

human g-csf
Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases. To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting. Revvity also worked with Myassays.com to help you in your data analysis.

Assay details

Technical specifications of human G-CSF kit
Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent) 27 pg/mL &47 pg/mL
Range 47  4,500 pg/mL
Time to result Overnight at RT
Calibration NIBSC (09/136) value (IU/mL) = 0,07 x HTRF hGCSF HTRF
Species Human only

 

Analytical performance

Intra and inter assay

Intra-assay (n=24)

Sample Mean [G-CSF] (pg/mL) CV
1 47 18%
2 2036 7%
3 3780 6%
  Mean CV 10%


Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample [G-CSF] (pg/mL) Mean (delta R) CV
1 87 222 13%
2 423 883 8%
3 2045 3161 6%
    Mean CV 9%


Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

Resources

1-3 of 3 Resources
Guide Icon
Guide
Cytokine assays: a guide to success with HTRF

The definitive guide to setting up a successful cytokine assay

Many therapeutic areas require an understanding of cytokine release...

Guide Icon
Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

Flyer Icon
Flyer
Reagent solutions for autoimmunity research.

Advance your autoimmune disease research and benefit from Revvity broad offering of reagent technologies

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

Scroll Icon